FastQCFastQC Report
Tue 11 Jun 2019
HKYKWAFXY_n02_CCPD51.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKYKWAFXY_n02_CCPD51.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30582078
Sequences flagged as poor quality0
Sequence length41
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAAAA20825396.809671337572287No Hit
ATACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAAAA19318746.3170135136010055No Hit
ATACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAAAA15235224.981747806672915No Hit
ATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAAAA13835464.52404182606558No Hit
ATACTCCTTTCAAGACCTAGCTAGCCAATTTGAATTCAAAA13800714.5126789618416385No Hit
ATACTCCTTTCAAGACCTAGCTAGCCTACATGAATTCAAAA13638134.459517106718517No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAAA12405464.056447701166677No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAAAA8530542.789391878472091No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAAGTCAAAA4678581.5298437208877695No Hit
ATACTCCTTTCAAGACCTAGCTAGCCATAAAGAAGTCAAAA4194811.371656301445572No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCATAA3743151.2239684955351955No Hit
ATACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCATAA3382151.1059255031656123No Hit
ATACTCCTTTCAAGACCTAGCTAGCACACGGGAAGTCAAAA3229581.0560368069167831No Hit
ATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAAGTCAAAA3109691.0168341078719372No Hit
ATACTCCTTTCAAGACCTAGCTAGCCTACATGAAGTCAAAA3010640.9844458574724713No Hit
ATACTCCTTTCAAGACCTAGCTAGCCAATTTGAAGTCAAAA3009480.9840665503501757No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAAGTCAAAA2825500.9239071327984972No Hit
ATACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCATAA2657070.8688323926189712No Hit
ATACTCCTTTCAAGACCTAGCTAGCCAATTTGAATTCATAA2456150.8031337831261827No Hit
ATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCATAA2427860.7938832671867491No Hit
ATACTCCTTTCAAGACCTAGCTAGCCTACATGAATTCATAA2395020.7831449517590008No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCATAA2101820.6872718067097991No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAAGTCAAAA1974690.645701708039591No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAAGTCATAA1820110.5951557641047152No Hit
ATACTCCTTTCAAGACCTAGCTAGCCATAAAGAAGTCATAA1609630.5263311407419732No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCATAA1431220.46799305135511066No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAAGTCAAGA1351720.44199743392191987No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCATGA1306630.42725350448717053No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAAGA1298170.42448717840560085No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAACA1288030.42117151097449945No Hit
ATACTCCTTTCAAGACCTAGCTAGCCATAAAGAAGTCAAGA1282360.4193174839198305No Hit
ATACTCCTTTCAAGACCTAGCTAGCACACGGGAAGTCATAA1261880.41262075127792164No Hit
ATACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAAGA1217500.39810898396112915No Hit
ATACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAACA1210430.3957971724485171No Hit
ATACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCATGA1201930.39301776681100614No Hit
ATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAAGTCATAA1171680.3831263526304524No Hit
ATACTCCTTTCAAGACCTAGCTAGCCAATTTGAAGTCATAA1160380.37943137807705546No Hit
ATACTCCTTTCAAGACCTAGCTAGCACACGGGAAGTCAAGA1140740.3730093161099125No Hit
ATACTCCTTTCAAGACCTAGCTAGCCTACATGAAGTCATAA1138870.3723978468696601No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAAGTCAATA1114230.3643408404098636No Hit
ATACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAAGA1108170.36235928768476755No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAATA1097470.358860506470489No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAAGTCAACA1082710.35403415032817587No Hit
ATACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCATGA1071090.3502345393272491No Hit
ATACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAATA1028580.336334241250709No Hit
ATACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAACA1020370.3336496623937719No Hit
ATACTCCTTTCAAGACCTAGCTAGCCATAAAGAAGTCAATA1017680.33277006225672434No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAAGTCATAA997560.32619104561828666No Hit
ATACTCCTTTCAAGACCTAGCTAGCCATAAAGAAGTCAACA996920.325981772723227No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCATCA947810.3099233479163842No Hit
ATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAAGTCAAGA936620.3062643421418257No Hit
ATACTCCTTTCAAGACCTAGCTAGCCAATTTGAATTCAAGA931020.3044332043100537No Hit
ATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAAGA929000.3037726867350217No Hit
ATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAACA929000.3037726867350217No Hit
ATACTCCTTTCAAGACCTAGCTAGCCTACATGAATTCAAGA926910.30308927993709256No Hit
ATACTCCTTTCAAGACCTAGCTAGCCAATTTGAAGTCAAGA922150.3015328127800864No Hit
ATACTCCTTTCAAGACCTAGCTAGCCTACATGAAGTCAAGA918720.3004112408581261No Hit
ATACTCCTTTCAAGACCTAGCTAGCCAATTTGAATTCATGA903810.29553583638103337No Hit
ATACTCCTTTCAAGACCTAGCTAGCCTACATGAATTCAACA896950.29329269253711276No Hit
ATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCATGA889310.29079449735233814No Hit
ATACTCCTTTCAAGACCTAGCTAGCCTACATGAATTCATGA887510.2902059173349829No Hit
ATACTCCTTTCAAGACCTAGCTAGCCAATTTGAATTCAACA876860.28672348556563093No Hit
ATACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCATCA860220.2812823902940801No Hit
ATACTCCTTTCAAGACCTAGCTAGCACACGGGAAGTCAACA845490.27646584381872286No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAACA810190.26492313570058906No Hit
ATACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAATA792710.25920736975427244No Hit
ATACTCCTTTCAAGACCTAGCTAGCACACGGGAAGTCAATA789800.25825583205954805No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCATAG776330.2538512915963395No Hit
ATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAAGTCAACA774450.2532365524671018No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAAGTCAAGA765040.2501595869319279No Hit
ATACTCCTTTCAAGACCTAGCTAGCCAATTTGAATTCAATA749460.24506509989281958No Hit
ATACTCCTTTCAAGACCTAGCTAGCGAATTCAAAAAAGCAC748890.24487871622065707No Hit
ATACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCATAG740400.2421025804721314No Hit
ATACTCCTTTCAAGACCTAGCTAGCCTACATGAAGTCAACA737690.24121644055711322No Hit
ATACTCCTTTCAAGACCTAGCTAGCCAATTTGAAGTCAATA737150.24103986655190662No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAAGTCATGA728850.2383258586941018No Hit
ATACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCATCA720980.2357524560626652No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAGA720010.23543527683109042No Hit
ATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAAGTCAATA718830.2350494299308242No Hit
ATACTCCTTTCAAGACCTAGCTAGCCTACATGAATTCAATA711200.23255450463503494No Hit
ATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAATA710640.23237139085185773No Hit
ATACTCCTTTCAAGACCTAGCTAGCCAATTTGAAGTCAACA709650.23204767184231237No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCATGA704790.23045850579545313No Hit
ATACTCCTTTCAAGACCTAGCTAGCCTACATGAAGTCAATA696150.2276333217121479No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAAGTCATAA688470.22512204697143207No Hit
ATACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCATAG683440.22347729281182266No Hit
ATACTCCTTTCAAGACCTAGCTAGCCATAAAGAAGTCATGA674540.22056709161489943No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAAGTCAACA673500.2202270231604275No Hit
ATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCATCA651580.2130594265046345No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCATAT637450.2084390733683957No Hit
ATACTCCTTTCAAGACCTAGCTAGCCTACATGAATTCATCA634040.207324041224406No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCATTA629400.20580681273522355No Hit
ATACTCCTTTCAAGACCTAGCTAGCCAATTTGAATTCATCA627750.2052672810526479No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAATA609580.19932589276634502No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAAGTCAATA607600.19867845474725426No Hit
ATACTCCTTTCAAGACCTAGCTAGCACACGGGAAGTCATGA596260.1949704006379161No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAACA575870.18830309699687509No Hit
ATACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCATTA561880.18372852230643058No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCATCA558650.1826723481641764No Hit
ATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCATAG556250.18188757480770273No Hit
ATACTCCTTTCAAGACCTAGCTAGCCAATTTGAATTCATAG554820.18141998068280382No Hit
ATACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCATAT552860.18077908244168364No Hit
ATACTCCTTTCAAGACCTAGCTAGCCTACATGAATTCATAG550670.1800629767539014No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAAGTCAAGA527960.1726370588682692No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAAGA506080.16548254176841742No Hit
ATACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAAAC498990.16316419047783476No Hit
ATACTCCTTTCAAGACCTAGCTAGCCAATTTGAAGTCATGA498490.1630006960285694No Hit
ATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAAGTCATGA497930.16281758224539222No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAAAC490210.16029322794873518No Hit
ATACTCCTTTCAAGACCTAGCTAGCCTACATGAAGTCATGA484360.15838034289233058No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCATGA481570.15746804386542995No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAAGTCAACA476240.1557251930362613No Hit
ATACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCATAT471610.15421123443606416No Hit
ATACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCATTA434460.1420635968556486No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAAGTCATTA433820.14185432396058895No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCATAC431540.14110878927193893No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCATAG429210.1403469051383624No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAAGTCATCA423750.13856154575238477No Hit
ATACTCCTTTCAAGACCTAGCTAGCCAATTTGAATTCATAT423730.13855500597441417No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAAGTCATAG420060.13735495671680648No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAAGTCAATA417020.13636091046527316No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAATA414040.13538648354765168No Hit
ATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCATAT413210.13511508276187117No Hit
ATACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCATAC411840.13466710797088413No Hit
ATACTCCTTTCAAGACCTAGCTAGCCAATTTGAATTCATTA406350.13287193891795057No Hit
ATACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAAAC401850.1314004888745624No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAAGTCAAAC396340.12959878004365824No Hit
ATACTCCTTTCAAGACCTAGCTAGCCATAAAGAAGTCATAG395250.1292423621442598No Hit
ATACTCCTTTCAAGACCTAGCTAGCCTACATGAATTCATAT394610.12903308924920015No Hit
ATACTCCTTTCAAGACCTAGCTAGCCATAAAGAAGTCAAAC391430.1279932645518725No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAAGTCATGA388470.1270253774122216No Hit
ATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCATCA386460.12636812972617492No Hit
ATACTCCTTTCAAGACCTAGCTAGCCTACATGAATTCATTA385980.12621117505488016No Hit
ATACTCCTTTCAAGACCTAGCTAGCCATAAAGAAGTCATTA385870.1261752062760418No Hit
ATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCATTA382330.12501766557524313No Hit
ATACTCCTTTCAAGACCTAGCTAGCCATAAAGAAGTCATCA378020.1236083434225758No Hit
ATACTCCTTTCAAGACCTAGCTAGCACACGGGAAGTCATAG378020.1236083434225758No Hit
ATACTCCTTTCAAGACCTAGCTAGCCTACATGAATTCAAAC358730.11730072756991856No Hit
ATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAAAC358570.11724840934615366No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAAAT347410.11359921323855103No Hit
ATACTCCTTTCAAGACCTAGCTAGCCAATTTGAATTCAAAC342260.11191522041111791No Hit
ATACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCATAC335230.10961648845444708No Hit
ATACTCCTTTCAAGACCTAGCTAGCGAATTCAAAAAAAAGA331390.10836085108408919No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAAGTCATAT328110.10728832749690848No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCATAT326900.10689267092968634No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCATTA325810.10653625303028787No Hit
ATACTCCTTTCAAGACCTAGCTAGCTCTGTAGAAGTCAAAT325390.10639891769290498No Hit
ATACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAAAT324990.1062681221334927No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAAC319340.10442063485679423No Hit
ATACTCCTTTCAAGACCTAGCTAGCACACGGGAAGTCAAAC317820.10392361173102757No Hit
ATACTCCTTTCAAGACCTAGCTAGCACACGGGAAGTCATCA315910.10329906293483392No Hit
ATACTCCTTTCAAGACCTAGCTAGCCAATTTGAAGTCATAG309580.10122922320713458No Hit
ATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAAGTCATAG307810.10065045285673525No Hit
ATACTCCTTTCAAGACCTAGCTAGCCTACATGAAGTCATAG306190.1001207308411155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTCA454.429239E-935.0716481
AAACTGC251.6910561E-435.0716481
AAACTCT251.6910561E-435.0716481
AAACTCG251.6910561E-435.0716481
TTAGGCC454.429239E-935.0716481
GGGCTCC750.035.0716481
ATAGGCG406.148548E-835.0716481
ATAATCC24050.035.0716481
ATAATAC650.035.0716481
GAACTCC2000.035.0716481
ACTCTCC358.5713873E-735.0716481
ATTCGCC1150.035.0716481
TAAGTCG200.002396241535.0716481
AGGGTCG200.002396241535.0716481
TTCCGCC454.429239E-935.0716481
TACGGCA200.002396241535.0716481
AATATCC301.2006254E-535.0716481
ATCGTCA503.2014214E-1035.0716481
ATACTCG3000.035.0716481
ATACTCA3950.035.0716481
Produced by FastQC (version 0.11.5)