FastQCFastQC Report
Tue 11 Jun 2019
HKYKWAFXY_n02_CCPD50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKYKWAFXY_n02_CCPD50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36154925
Sequences flagged as poor quality0
Sequence length41
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAAAAA27826597.696486716540001No Hit
TACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAAAA25565327.071047720331324No Hit
TACTCCTTTCAAGACCTAGCTAGCCTACATGAATTCAAAAA23570486.5192999294010425No Hit
TACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAAAAA19844825.488828976965102No Hit
TACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAAAAA17960094.9675362346900185No Hit
TACTCCTTTCAAGACCTAGCTAGCCAATTTGAATTCAAAAA17943954.963072112582172No Hit
TACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAAAAA14239903.9385782158309No Hit
TACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAAAAA9328722.580207260836525No Hit
TACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAATAA4610211.275126417770193No Hit
TACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAATAA4275191.1824640764709096No Hit
TACTCCTTTCAAGACCTAGCTAGCCTACATGAATTCAATAA3837641.0614432196996675No Hit
TACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAATAA3414960.9445352189224565No Hit
TACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAAACA3051430.8439873682492773No Hit
TACTCCTTTCAAGACCTAGCTAGCCAATTTGAATTCAATAA2906610.8039319677747915No Hit
TACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAATAA2826310.7817219922320403No Hit
TACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAAAGA2815970.7788620775731108No Hit
TACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAACA2815940.7788537799483749No Hit
TACTCCTTTCAAGACCTAGCTAGCCTACATGAATTCAAACA2653020.7337921458833063No Hit
TACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAAGA2634130.7285674081746816No Hit
TACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAATAA2505030.6928599630617405No Hit
TACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAAAAC2463870.6814756219242607No Hit
TACTCCTTTCAAGACCTAGCTAGCCTACATGAATTCAAAGA2328990.6441695011122275No Hit
TACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAAAC2297820.6355482690117599No Hit
TACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAAACA2179280.6027615878058107No Hit
TACTCCTTTCAAGACCTAGCTAGCCTACATGAATTCAAAAC2161700.597899179710648No Hit
TACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAAAGA2086730.5771634154959525No Hit
TACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAATAG2033290.5623825799666298No Hit
TACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAAACA2021470.5591133158207353No Hit
TACTCCTTTCAAGACCTAGCTAGCCAATTTGAATTCAAACA1931790.5343089496105994No Hit
TACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAATGA1922680.5317892375658365No Hit
TACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAATAG1921510.5314656302011413No Hit
TACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAATGA1788410.4946518351234306No Hit
TACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAAAAC1774630.4908404594947991No Hit
TACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAAAGA1688680.4670677646268108No Hit
TACTCCTTTCAAGACCTAGCTAGCCAATTTGAATTCAAAGA1682990.465493981801926No Hit
TACTCCTTTCAAGACCTAGCTAGCCTACATGAATTCAATAG1666000.46079476032656685No Hit
TACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAAATA1646860.45550087574514403No Hit
TACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAAAAC1617800.4474632432510924No Hit
TACTCCTTTCAAGACCTAGCTAGCCAATTTGAATTCAAAAC1565290.4329396340885785No Hit
TACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAATAA1551940.4292471910811598No Hit
TACTCCTTTCAAGACCTAGCTAGCCTACATGAATTCAATGA1551190.42903975046276543No Hit
TACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAATAG1550250.4287797582210446No Hit
TACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAATA1532750.4239394771251773No Hit
TACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAATCA1502090.4154593046452178No Hit
TACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAAACA1499770.4148176216656514No Hit
TACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAAAGA1481900.4098750031980429No Hit
TACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAATGA1444650.3995721191511254No Hit
TACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAATCA1401700.387692686404411No Hit
TACTCCTTTCAAGACCTAGCTAGCCTACATGAATTCAAATA1364850.37750043735397043No Hit
TACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAATAC1351540.3738190578461994No Hit
TACTCCTTTCAAGACCTAGCTAGCCTACATGAATTCAATCA1294360.358003785099817No Hit
TACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAATAC1291420.35719061787571127No Hit
TACTCCTTTCAAGACCTAGCTAGCACACGGGAATGCAAAAA1217580.33676739752606316No Hit
TACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAAATA1203140.33277347415324465No Hit
TACTCCTTTCAAGACCTAGCTAGCCAATTTGAATTCAATAG1197420.33119139370362405No Hit
TACTCCTTTCAAGACCTAGCTAGCCTACATGAATTCAATAC1183250.32727214895342754No Hit
TACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAAAAC1173340.32453116691571066No Hit
TACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAAAAG1168020.323059721462567No Hit
TACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAATAG1128520.3121345155604665No Hit
TACTCCTTTCAAGACCTAGCTAGCCAATTTGAATTCAATGA1127520.3118579280692741No Hit
TACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAATCA1123460.31073498285503287No Hit
TACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAAAG1100830.3044758079293485No Hit
TACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAATGA1098240.3037594463271601No Hit
TACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAATAG1091940.3020169451326479No Hit
TACTCCTTTCAAGACCTAGCTAGCCAATTTGAATTCAAATA1064400.2943997256252088No Hit
TACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAAATA1062810.2939599515142128No Hit
TACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAATGA1044580.288917761549775No Hit
TACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAATAC1043420.2885969200599918No Hit
TACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAAACA1034280.28606891039049315No Hit
TACTCCTTTCAAGACCTAGCTAGCTTGGACGAATGCAAAAA1002280.2772181106723358No Hit
TACTCCTTTCAAGACCTAGCTAGCCTACATGAATTCAAAAG955630.26431530420820953No Hit
TACTCCTTTCAAGACCTAGCTAGCCAATTTGAATTCAATCA947700.26212196540305366No Hit
TACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAATCA947570.26208600902919865No Hit
TACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAAAGA936810.25910992762396823No Hit
TACTCCTTTCAAGACCTAGCTAGCCTACATGAATGCAAAAA933530.25820272065285715No Hit
TACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAAATA903330.24984977841884612No Hit
TACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAAAAG853430.2360480626083445No Hit
TACTCCTTTCAAGACCTAGCTAGCCAATTTGAATTCAATAC849070.2348421411467456No Hit
TACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAATAC823240.22769788624924545No Hit
TACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAAAAC821260.22715024301668443No Hit
TACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAATCA787680.21786243506244307No Hit
TACTCCTTTCAAGACCTAGCTAGCCAATTTGAATGCAAAAA783610.21673672397328997No Hit
TACTCCTTTCAAGACCTAGCTAGCACACGGGAATGCAAAGA773360.2139017021885677No Hit
TACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATGCAAAAA741900.20520025971565423No Hit
TACTCCTTTCAAGACCTAGCTAGCCATAAAGAATGCAAAAA736560.20372328251268673No Hit
TACTCCTTTCAAGACCTAGCTAGCACACGGGAATGCAAACA722140.19973489088969207No Hit
TACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAATAC708350.19592074938614865No Hit
TACTCCTTTCAAGACCTAGCTAGCCAATTTGAATTCAAAAG706410.19538416965323535No Hit
TACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAAAAG687820.1902424081919683No Hit
TACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAATTA681430.18847501412324877No Hit
TACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAATAG654740.18109289398332315No Hit
TACTCCTTTCAAGACCTAGCTAGCTTGGACGAATGCAAAGA641340.17738662160134477No Hit
TACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAATGA628250.17376609134163604No Hit
TACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAATTA623490.17244953488356013No Hit
TACTCCTTTCAAGACCTAGCTAGCTTGGACGAATGCAAACA603340.16687629693603293No Hit
TACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAAAAG583090.16127540023938647No Hit
TACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAAATA567270.15689978612872243No Hit
TACTCCTTTCAAGACCTAGCTAGCCTACATGAATGCAAACA554740.15343414486408144No Hit
TACTCCTTTCAAGACCTAGCTAGCCTACATGAATTCAATTA552390.15278416425977928No Hit
TACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAAAGC549740.15205120740811937No Hit
TACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATGCAAAAA548400.15168058016992153No Hit
TACTCCTTTCAAGACCTAGCTAGCCTACATGAATGCAAAGA541520.1497776582305177No Hit
TACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAAGC524490.14506737325551083No Hit
TACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAATTA499860.1382550233474416No Hit
TACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAATCA497750.1376714237410256No Hit
TACTCCTTTCAAGACCTAGCTAGCCATAAAGAATGCAAAGA485450.13426939759935888No Hit
TACTCCTTTCAAGACCTAGCTAGCCTACATGAATTCAAAGC460160.12727449994710266No Hit
TACTCCTTTCAAGACCTAGCTAGCCATAAAGAATGCAAACA452870.12525817713630993No Hit
TACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAATAC450260.12453628378429771No Hit
TACTCCTTTCAAGACCTAGCTAGCCAATTTGAATGCAAAGA432100.11951345494424342No Hit
TACTCCTTTCAAGACCTAGCTAGCCAATTTGAATGCAAACA431590.11937239532373528No Hit
TACTCCTTTCAAGACCTAGCTAGCCAATTTGAATTCAATTA430570.11909027608271903No Hit
TACTCCTTTCAAGACCTAGCTAGCACACGGGAATGCAATAA420360.11626631779764443No Hit
TACTCCTTTCAAGACCTAGCTAGCCATAAAGAATTCAAAGC417130.11537294020109294No Hit
TACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATGCAAACA416460.11518762658199401No Hit
TACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAATTA415490.11491933671553738No Hit
TACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAATAT407170.11261812878881647No Hit
TACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATGCAAAGA398920.11033628198647903No Hit
TACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAATGC398520.11022564699000206No Hit
TACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAATGC392050.1084361259219871No Hit
TACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAAAAT386620.10693425584481228No Hit
TACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAAACC385100.10651384285819981No Hit
TACTCCTTTCAAGACCTAGCTAGCTCTGTAGAATTCAATTA384060.1062261918673597No Hit
TACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAAAAG379830.10505622677961578No Hit
TACTCCTTTCAAGACCTAGCTAGCCGACCCGAATGCAAAAA366280.10130846627395852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCCT21550.035.0691681
TACTTCT9850.035.0691681
TAATACT1450.035.0691641
ATCTCCT1250.035.0691641
GTCGCCC200.00239707935.0691641
GTCTGCC200.00239707935.0691641
AGATACT503.2014214E-1035.0691641
TCCTGCT552.3646862E-1135.0691641
AACTTCT503.2014214E-1035.0691641
TAAACCC800.035.0691641
TTCGTCT200.00239707935.0691641
TACGGCT9650.035.0691641
TTATCCT251.6917664E-435.0691641
AACGGCT1950.035.0691641
TACGCCC2100.035.0691641
TACGCCA750.035.0691641
ATATACT251.6917664E-435.0691641
TACTGCT51250.035.0691641
TACTGCA503.2014214E-1035.0691641
TGCTGCT251.6917664E-435.0691641
Produced by FastQC (version 0.11.5)