FastQCFastQC Report
Tue 11 Jun 2019
HKYKWAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKYKWAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10665607
Sequences flagged as poor quality0
Sequence length71
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG412020038.630712719866764No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG626300.5872145860990378No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG229900.064.789251
TCGTTAC218000.064.7647555
TCGATCG217700.064.716851
TCGATTC233200.064.710622
TCCTTGG232600.064.683467
ATCGCGC160350.064.678992
AGGTCTT231450.064.5749415
ATCGATT233500.064.5143051
TACACGA201550.064.5033952
TCGCATC92500.064.4989857
CACGATC203600.064.460544
GATCGCG160100.064.446971
GATTCCT234500.064.435044
TAAGGTC232450.064.3820413
ATTCCTT234750.064.338745
GTATCTC97050.064.33356
GATCTCT207250.064.31667
CATGATC110550.064.297715
GGTATTC195550.064.2928712
CGATAGG233100.064.29226
Produced by FastQC (version 0.11.5)