FastQCFastQC Report
Tue 11 Jun 2019
HKYKWAFXY_n01_CCPD53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKYKWAFXY_n01_CCPD53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15705805
Sequences flagged as poor quality0
Sequence length71
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGTTTAAGAGCTATGCT361160.22995319246609772No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGTTTAAGAGCTATGC327490.2085152591669131No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGCTCGGCCGCGCGACGGTCAGTTTAAGAGC245290.15617792274894537No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCGCGCGGCCGAGCGTGCCCGGTTTAAGAGC201990.12860849857743684No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGGGCACGCTCGGCCGCGCGAGTTTAAGAGC184090.11721143870053143No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGTTTAAGAGCTATGCTG180100.1146709767503162No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCGGCCGCGCGACGGTCATGGGTTTAAGAGC171600.10925896507692537No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGCGCGGCCGAGCGTGCCCGCGTTTAAGAGC166130.10577617638828445No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGACCGCCCCGAGGCGCGCGCGGTTTAAGAGCT165640.10546418983299487No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCGCGGGCACGCTCGGCCGCGGTTTAAGAGCT165040.10508216547957905No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGACCGCCCCGAGGCGCGCGCGGTTTAAGAGC164710.10487205208520034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG6472500.065.014311
ATACAGG6031750.064.837067
TCGATAG6530300.064.827555
CGATAAC2920450.064.799644
CGATAGG6523650.064.797976
TCGATAC6050750.064.7914354
ATAACGC2921700.064.787496
CAGGTAT6023500.064.77209510
AACGCAT2924800.064.7459268
GGTATTC6022900.064.7292312
TCGATAA2923450.064.72073
TAAGGTC6541550.064.7192413
ACAGGTA6038850.064.718859
TAACGCA2922800.064.717967
ACGCATT2923400.064.7124569
GATACAG6055700.064.710026
CGATACA6057650.064.708515
TAGGTAA6551800.064.7035149
AAGGTCT6553150.064.6894814
AGGTCTT6548550.064.6421215
Produced by FastQC (version 0.11.5)