FastQCFastQC Report
Tue 11 Jun 2019
HKYKWAFXY_n01_CCPD52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKYKWAFXY_n01_CCPD52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30269947
Sequences flagged as poor quality0
Sequence length71
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGTCGGGGTTTGAAAC1927140.636651263380144No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTTTTCTGATGCTACGGATACGTTTTAGAGC1109420.3665087355455231No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGTAGTCCCCTCCTTATCTGCGTTTTAGAGC820310.2709981619723351No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGTTTAAGAGCTATGCTGGA461990.1526233263639345No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGGAGACGGACGTCTCTGTTTAAGAGCTATGCTGG459300.15173465615912707No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTTTCCATTGCAGGTATGTCTGTTTTAGAGC391520.12934280988334734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG6169050.064.990941
TCGATTC6709000.064.948612
TCGATCG5945450.064.89941
TCGTTAC5953300.064.876585
ATCGTTA5955050.064.8760764
CATGATC3473550.064.853685
ATAACGC2748950.064.822886
ATACAGG4742200.064.781667
ATCGATT6701850.064.7787861
TACCATC5984000.064.777089
CGATAGG6224200.064.7717066
CCATCTT5967950.064.7378211
TCGATAG6237950.064.733685
AACGCAT2753650.064.7325748
TAAGGTC6241550.064.7263213
TCCTTGG6723150.064.725117
TTGGTTC6727050.064.7188810
ATGATCG3478900.064.7165766
GATTCCT6741100.064.711944
TCGATAA2750750.064.698933
Produced by FastQC (version 0.11.5)