FastQCFastQC Report
Tue 11 Jun 2019
HKYKWAFXY_n01_CCPD51.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKYKWAFXY_n01_CCPD51.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30582078
Sequences flagged as poor quality0
Sequence length71
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGTTTTCTGATGCTACGGATACGTTTTAGAGCTAGA698710.2284704132923865No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGTCGGGGTTTGAAACGGAG531080.17365726423168498No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAGAAGCCAAACCTTTGAGATGTTTAAGAGCTATG391480.12800961399679905No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCTTCGACGCCATCGTGCTCAGTTTAAGAGCTATG311240.10177202477869554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGCG4289500.064.9971541
TCGATTC6057350.064.9715352
TACACGA5487150.064.964272
ATCGATT6046900.064.949851
TCGATCG6379750.064.943861
ATCGCGC4301400.064.9267042
ATCGTTA6393600.064.921424
TCGTTAC6396600.064.89915
ATCATGA3181900.064.898013
CACGATC5501450.064.880074
TCATGAT3201750.064.863184
ATGATCG3200400.064.837736
TCCTTGG6060150.064.809637
CATGATC3203650.064.791245
TAGAGTC4653150.064.77775
TTACCAT6430000.064.7704248
CCATCTT6409400.064.7570211
TCTCTTG5551800.064.752019
GATCTCT5518450.064.7375647
ACGATCT5520550.064.702125
Produced by FastQC (version 0.11.5)