FastQCFastQC Report
Tue 11 Jun 2019
HKYKWAFXY_n01_CCPD50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKYKWAFXY_n01_CCPD50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36154925
Sequences flagged as poor quality0
Sequence length71
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGCG4671950.065.01181
TACACGA6241700.064.977132
ATCGCGC4684700.064.961532
ATAGGTC6607400.064.9534452
AGGTCGC6599200.064.933134
ACTGTAT8250800.064.929313
CACGATC6258750.064.923194
GGTCGCA6595100.064.919785
TCGCATC6624350.064.882417
TAGAGTC4873550.064.871565
ACACGAT6264400.064.865063
AAGTAGA4870150.064.835962
TCTCTTG14608900.064.808039
AGTCTTG4892750.064.806728
GAGTCTT4868250.064.8065957
GATCTCT6286850.064.802277
TAACAAT4865600.064.792711
AGAGTCT4879000.064.7897646
TGGTCTT4837900.064.7812047
ATACACG6259550.064.776751
Produced by FastQC (version 0.11.5)