FastQCFastQC Report
Wed 13 Sep 2017
HKWY5BGX3_n01_tga1ox_60n_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKWY5BGX3_n01_tga1ox_60n_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13012874
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG188730.14503329548875982No Hit
GTTTTACCCTACTGATGCCCGCGTCGCGATAGTAATTCAACCTAGTACGAGAGGAACCGTTGATTCGCACAATTG132530.10184529566643002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG39750.035.0620548
TGTGTGC51900.027.5850547
TGTGCGA51950.026.828049
GTGCGAG53250.025.97872510
CTGACAT58800.025.7020841
GCCAGTA38450.025.5752581
TGCGAGT56400.024.34428411
CGGGCGC17500.023.6572715
ACATGTG61650.023.558024
ATGTGTG63100.023.0169356
GACATGT67150.022.296253
GCGAGTC65400.020.99407612
TGCCCGC46200.020.90944716
GGCGGGC20000.020.69947613
TGACATG70850.020.5478952
CCCTACT49650.019.5939927
ACGGGTG71950.019.4682420
ATGCCCG50350.019.18580415
CGGGTGA73750.018.8994521
ACCCGTA73300.018.82604632
Produced by FastQC (version 0.11.2)