FastQCFastQC Report
Wed 13 Sep 2017
HKWY5BGX3_n01_tga1ox_20n_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKWY5BGX3_n01_tga1ox_20n_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6443062
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG243150.37738267922922364No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAAACTG197170.3060190946478553No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGG168910.26215796154064636No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTACGTGGAGGCATCCCTTCAC160870.24967942260993298No Hit
GCGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGG150410.23344490554335812No Hit
GTTTTACCCTACTGATGCCCGCGTCGCGATAGTAATTCAACCTAGTACGAGAGGAACCGTTGATTCGCACAATTG148640.23069776451010404No Hit
CAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTTAG136250.2114677772773256No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAAACTGCGAATGG133760.2076031551457987No Hit
CCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGTCG116590.18095433506615333No Hit
CGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGC113010.17539797071640784No Hit
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCAACGT102820.15958250906168528No Hit
AAAGGATTCTACCCGCCGCTCGGTGGTAATTATAATTCAAGGCGGTCCGAACGACGCTTCCGCCGAACGGACTTA93950.14581576275379626No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGA90980.14120615322342078No Hit
GGATTCTACCCGCCGCTCGGTGGTAATTATAATTCAAGGCGGTCCGAACGACGCTTCCGCCGAACGGACTTAGCC89280.13856765618583214No Hit
GTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGGTGCACCGCC87030.13507552775372952No Hit
GTTAGTTTTACCCTACTGATGCCCGCGTCGCGATAGTAATTCAACCTAGTACGAGAGGAACCGTTGATTCGCACA86120.13366315581007912No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAAACTGCGAATGGCTCATTAA85960.13341482667712962No Hit
CTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCCGTATT82510.12806022974790557No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTAATTCTCCGTC76740.1191048603909135No Hit
CTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTAATTCTCCGTCACCCGTTACCACCAT76480.11870132554987055No Hit
GTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAAACTGCGAA73390.11390546916978295No Hit
CCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGT72050.11182571268133071No Hit
GTCGGCTTGTCCCTTCGGTCGGCGATACGCTCCTGGTCTTAATTGGCCGGGTCGTGCCTCCGGCGCTGTTACTTT71590.11111176642410084No Hit
GAAATTTCAAAGATTACCCGGGCCTGTCGGCCAAGGTGTGAACTCGTTGAATACATCAGTGTAGCGCGCGTGCGG70780.1098546001885439No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT70290.10909409221888598No Hit
TATAATTCAAGGCGGTCCGAACGACGCTTCCGCCGAACGGACTTAGCCAACGACACGTGCCTTTGGGAGCCTAAG69290.1075420351379515No Hit
CAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAAACTGCGAATGGCTCATTAAATCAGTT66240.10280826104110126No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTTAGAGGCGTTCAGTCATAATCCAGC65770.10207879421306204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA32300.044.015521
CTGACAT39600.041.9141851
GTGTGCG42050.038.723018
TGTGTGC44850.036.767037
ACATGTG46250.035.8032654
TGACATG46300.035.5416222
GACATGT47450.034.9705123
CCAGTAG42000.033.841362
ATGTGTG50150.033.087756
TGTGCGA50400.032.444499
GTGCGAG52850.030.87516210
TGCGAGT54850.029.81248511
CAGTAGT47700.029.7246383
AGTAGTC50300.028.1195894
GGCGGGC18600.026.15206713
CGGGCGC18750.025.75925615
GCGAGTC65950.024.74245612
GGGATTA46000.024.6735233
CCCTGGT39850.024.33017567
ACATCCG40850.023.7332955
Produced by FastQC (version 0.11.2)