FastQCFastQC Report
Wed 13 Sep 2017
HKWY5BGX3_n01_tga1ox_20n_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKWY5BGX3_n01_tga1ox_20n_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12961007
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG141680.10931249400605986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG34850.031.9730688
TGTGCGA45200.024.8809579
TGTGTGC47500.024.5473337
CGGGCGC15300.024.35278915
GTGCGAG48500.023.04594610
CTGACAT54750.022.0579781
TGCGAGT50850.021.91330311
ACATGTG56250.021.1578274
ATGTGTG56700.020.9291486
GGCGGGC18250.020.60503413
GCCAGTA34550.020.5731561
GACATGT62050.019.8475073
GCGAGTC58150.019.16236512
TGCCCGC36900.018.79271116
CCCTACT38850.018.114257
ATGCCCG39650.017.92422715
TGACATG65450.017.6570452
CGCGCGT21150.017.61976665
ACCCGTA63250.017.5628532
ACGGGTG63300.017.55012920
Produced by FastQC (version 0.11.2)