FastQCFastQC Report
Wed 13 Sep 2017
HKWY5BGX3_n01_tga1ox_10n_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKWY5BGX3_n01_tga1ox_10n_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27944338
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG61600.031.6976858
CGGGCGC46250.027.89777615
GGCGGGC50100.025.96014413
TGTGCGA87200.022.6688739
ATCGGGC61350.022.5486686
TGTGTGC90600.022.4654777
GTGCGAG89450.021.71300110
TCGGGCG61100.021.5682037
TGCGAGT94300.020.55972111
GCCAGTA67950.020.1592181
GCGGGCG64100.020.1287614
TAGCCCG65200.019.7892734
GGCGCAT66800.019.4192317
ACATGTG113000.018.9278774
CTGACAT116600.018.9092791
CGGCAAT89000.018.6478041
TGCCCGC85850.018.24444616
GCGAGTC108800.018.07334512
ATGTGTG118800.017.742546
CCCTACT90100.017.6126567
Produced by FastQC (version 0.11.2)