FastQCFastQC Report
Wed 13 Sep 2017
HKWY5BGX3_n01_tga1ox_05n_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKWY5BGX3_n01_tga1ox_05n_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22835456
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG292610.12813845276398247No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAAACTG249740.10936501552673177No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG65200.033.5452238
TGTGTGC84000.026.4070997
GCCAGTA67350.026.1278881
TGTGCGA86750.025.3313879
CTGACAT98750.024.423721
GTGCGAG89200.024.36495610
TGCGAGT91150.023.76817311
CGGGCGC30450.023.22626515
ACATGTG104000.022.389984
ATGTGTG109300.021.5884826
GACATGT109850.021.4174423
GGCGGGC34550.020.66960513
TGACATG112150.020.5169452
GCGAGTC108800.019.94410512
TGCCCGC76450.019.35981216
CGCGCGT41100.018.7214165
ACGGGTG117350.018.61005620
CCCTACT82200.018.4239067
CGGGTGA118800.018.38287221
ATGCCCG81900.018.19753815
Produced by FastQC (version 0.11.2)