FastQCFastQC Report
Wed 13 Sep 2017
HKWY5BGX3_n01_tga1ox_01n_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKWY5BGX3_n01_tga1ox_01n_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8549601
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG182110.21300409223775474No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGG153700.17977447134667454No Hit
GTTTTACCCTACTGATGCCCGCGTCGCGATAGTAATTCAACCTAGTACGAGAGGAACCGTTGATTCGCACAATTG146480.17132963280976504No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAAACTGCGAATGG133590.15625290583736012No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAAACTG127570.14921164157251315No Hit
CAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTTAG119430.13969072942702238No Hit
CGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGC115060.13457937978626136No Hit
GCGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGG113430.13267285806670978No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAAACTGCGAATGGCTCATTAA108390.1267778461240472No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGA102120.11944417055252052No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTACGTGGAGGCATCCCTTCAC98810.1155726448520814No Hit
CCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGTCG98380.11506969740459232No Hit
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCAACGT96510.11288246083062824No Hit
GTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGGTGCACCGCC88120.10306913737845777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG37200.037.279788
TGTGTGC41450.033.7070477
CTGACAT43450.033.1144681
GCCAGTA26250.032.9925541
ACATGTG45200.031.3683074
TGTGCGA45800.030.2043239
GACATGT47600.030.0041313
ATGTGTG48900.029.2771956
GTGCGAG48350.028.46863410
TGACATG50650.027.5843812
TGCGAGT52100.026.41954811
GGCGGGC21150.025.93581213
CGGGCGC22450.024.43481815
ATGCCCG47300.023.48675715
TGCCCGC47700.023.36254316
CCAGTAG37300.022.9366782
CCCTACT49600.022.6738977
GCGAGTC62750.022.10039512
CGCGCGT23750.022.0822865
TACCCTA52600.021.2495385
Produced by FastQC (version 0.11.2)