FastQCFastQC Report
Wed 13 Sep 2017
HKWY5BGX3_n01_tga1ox_01n_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKWY5BGX3_n01_tga1ox_01n_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12780312
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAAACTG143640.11239162236414886No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTACGTGGAGGCATCCCTTCAC139350.10903489680064149No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG135640.10613199427369222No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG31600.029.803818
GCCAGTA38500.024.8256281
CGGGCGC15200.022.01657915
GTGCGAG43200.021.96099310
TGTGTGC44250.021.829367
TGTGCGA44100.021.8256369
GGCGGGC16100.020.78543313
TGCGAGT47150.019.9748711
CGCGCGT22350.019.14285365
CTGACAT55450.018.9792671
CGGGATT49000.018.727442
GCTACGT56000.018.54393653
TACGTGG56200.018.3558155
ACATGTG57200.018.3344844
GCGATTT65550.018.1078511
ACCCGCT46500.017.8799139
GCGCGCG24100.017.75281164
GACATGT61000.017.7013193
GCGAGTC54950.017.2649912
CAGTAGT56950.017.2034573
Produced by FastQC (version 0.11.2)