FastQCFastQC Report
Wed 13 Sep 2017
HKWY5BGX3_n01_col_60n_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKWY5BGX3_n01_col_60n_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11353727
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG117570.10355189974182047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG28550.030.0872028
TGTGCGA38100.022.545869
TGCGAGT38850.022.11090511
GTGCGAG39050.021.99756210
TGTGTGC40850.021.8723957
CTGACAT49450.019.5367811
TGCCCGC28800.019.52647616
GCGAGTC44500.019.2260412
ACCCGTA45250.019.2124232
ACATGTG52050.018.6240524
CGGGCGC7800.018.57725515
ACGGGTG46750.018.37564820
ATGTGTG52850.018.3421386
CCCGTAA48850.017.72593933
CGGGTGA48950.017.54977821
AGGCGCA50200.017.5236739
CCCTACT33000.017.3533277
TAAGGCG49450.017.3013437
GACATGT56250.017.233383
GTTAGTT27800.016.8793371
Produced by FastQC (version 0.11.2)