FastQCFastQC Report
Wed 13 Sep 2017
HKWY5BGX3_n01_col_60n_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKWY5BGX3_n01_col_60n_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18483792
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG293950.15903122043355605No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTACGTGGAGGCATCCCTTCAC266150.14399101656196955No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGG253240.13700651900865363No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAAACTG232750.12592113133495553No Hit
GCGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGG218610.11827118591250106No Hit
CAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTTAG194370.10515699375972204No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAAACTGCGAATGG194010.10496222852973025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG61000.033.366588
GCCAGTA56850.029.7399181
TGTGTGC77650.026.8784037
TGTGCGA77250.026.526359
CTGACAT81850.026.5158841
GTGCGAG78200.026.07196610
TGCGAGT84500.024.12820611
GACATGT97600.022.4089743
ACATGTG96350.022.1626384
CGGGCGC26150.021.63658715
TGACATG99300.021.2959042
CGCGCGT39500.020.87732965
ATGTGTG103900.020.6850856
CCAGTAG81150.020.6601092
GGCGGGC27900.020.27939613
GCGAGTC101550.020.2471612
CAGTAGT86650.019.4680653
GCGATTT106700.019.3056341
GTAGCGC44800.019.02387461
GGGATTA84000.018.7269943
Produced by FastQC (version 0.11.2)