FastQCFastQC Report
Wed 13 Sep 2017
HKWY5BGX3_n01_col_20n_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKWY5BGX3_n01_col_20n_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5781338
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG71770.12414081307821823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG15000.033.1186528
CGGGCGC5400.025.5556215
TGTGTGC21150.025.1195457
GCCAGTA15550.024.8540951
GTGCGAG20300.024.47233410
TGTGCGA20800.024.0497119
TGCGAGT21200.023.27068111
CGCGCGT8100.023.00125365
CTGACAT23650.022.6157441
GCGAGTC24250.020.62838412
ATGTGTG27000.020.3158386
GTAGCGC9450.020.0804661
GGCGGGC6900.019.99987613
GCGCGCG9400.019.82022764
ACATGTG28050.019.5553534
GACATGT29250.019.2248573
ACGGGTG25900.019.1816820
TGCCCGC15700.019.11771616
CGGGTGA26900.018.34019321
TGACATG29200.018.3126092
Produced by FastQC (version 0.11.2)