FastQCFastQC Report
Wed 13 Sep 2017
HKWY5BGX3_n01_col_20n_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKWY5BGX3_n01_col_20n_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15470421
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG243430.15735189107006203No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGG182440.11792827098887614No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTACGTGGAGGCATCCCTTCAC181390.11724955642771454No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAAACTG171950.1111475893254618No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAAACTGCGAATGG166100.10736617962756152No Hit
GTTTTACCCTACTGATGCCCGCGTCGCGATAGTAATTCAACCTAGTACGAGAGGAACCGTTGATTCGCACAATTG157710.10194292708647036No Hit
CAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTTAG157470.10178779232963343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG50700.034.2936638
TGTGTGC60850.028.8001277
GCCAGTA45900.026.9869881
TGTGCGA65500.026.7028689
CTGACAT71250.026.247451
GTGCGAG66750.025.94457410
TGCGAGT73450.023.57794811
ACATGTG78300.023.394754
ATGTGTG79250.022.8533546
GACATGT83350.022.5567513
CGGGCGC19500.021.93861615
CGCGCGT28150.021.81824965
TGACATG85250.021.6901072
GCGAGTC83550.021.09925512
TGCCCGC61150.019.6906916
GGCGGGC22500.019.31982213
CCCTACT64650.019.0498247
ATGCCCG65450.018.81831415
GTAGCGC33250.018.57498461
GCGCGCG33500.018.33384364
Produced by FastQC (version 0.11.2)