FastQCFastQC Report
Wed 13 Sep 2017
HKWY5BGX3_n01_col_10n_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKWY5BGX3_n01_col_10n_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22613647
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAAACTG686920.30376347521476743No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG677890.2997703112638134No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGG557000.2463114419359248No Hit
GCGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGG535830.23694983829897054No Hit
GTTTTACCCTACTGATGCCCGCGTCGCGATAGTAATTCAACCTAGTACGAGAGGAACCGTTGATTCGCACAATTG476960.21091688571949493No Hit
GTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAAACTGCGAATGG429560.18995609155834087No Hit
CGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGC372620.16477660591411902No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTACGTGGAGGCATCCCTTCAC368350.16288836559622602No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTTAGAGGCGTTCAGTCATAATCCAGC340000.15035168807578891No Hit
TATAATTCAAGGCGGTCCGAACGACGCTTCCGCCGAACGGACTTAGCCAACGACACGTGCCTTTGGGAGCCTAAG339370.15007309524200144No Hit
CCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAGCGGAGTCCTATAAGCAACATCCGCTGATCCCTGGTCG325490.143935208681731No Hit
CAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTTAG314710.13916817574803392No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTAATTCTCCGTC311290.1376558146503304No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGGAAGA298420.1319645610458145No Hit
GTTAGTTTTACCCTACTGATGCCCGCGTCGCGATAGTAATTCAACCTAGTACGAGAGGAACCGTTGATTCGCACA286470.12668014142079778No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAAACTGCGAATGGCTCATTAA284600.12585320713638098No Hit
TTATAATTCAAGGCGGTCCGAACGACGCTTCCGCCGAACGGACTTAGCCAACGACACGTGCCTTTGGGAGCCTAA271200.11992758178280576No Hit
GTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGGTGCACCGCC253580.11213582665370164No Hit
CCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGT252960.11186165592838697No Hit
GTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAAACTGCGAA244700.10820899432983984No Hit
GAAATTTCAAAGATTACCCGGGCCTGTCGGCCAAGGTGTGAACTCGTTGAATACATCAGTGTAGCGCGCGTGCGG242120.10706809034385298No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTAATTCTCCGT238320.10538768912418241No Hit
CTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTAATTCTCCGTCACCCGTTACCACCAT236360.1045209558635102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAGTA117350.040.5768361
CTGACAT133600.038.843961
GTGTGCG132450.038.3398678
TGTGTGC142850.035.8624577
ACATGTG152800.033.9333344
TGACATG153500.033.6438942
GACATGT159300.032.8519173
ATGTGTG162950.031.8833856
TGTGCGA162900.031.2579869
CCAGTAG154100.030.848882
GTGCGAG164650.030.77909310
TGCGAGT175650.028.87133211
GGCGGGC82450.028.57848513
CAGTAGT172700.027.6261753
CGGGCGC87000.027.0443915
TCGGGCG91100.026.3941827
ATCGGGC93400.026.1135756
CGGGATT158650.026.0283682
AGTAGTC182700.025.944134
GGGATTA164550.024.8643863
Produced by FastQC (version 0.11.2)