FastQCFastQC Report
Wed 13 Sep 2017
HKWY5BGX3_n01_col_10n_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKWY5BGX3_n01_col_10n_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7765773
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG142830.1839224504759539No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGG97650.125744082398494No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAAACTG90450.11647262931842071No Hit
GTTTTACCCTACTGATGCCCGCGTCGCGATAGTAATTCAACCTAGTACGAGAGGAACCGTTGATTCGCACAATTG90090.11600905666441705No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTACGTGGAGGCATCCCTTCAC81410.10483180489566203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG30650.036.0174188
CTGACAT37350.030.209041
TGTGCGA37000.029.6495749
TGTGTGC38400.029.3771467
CGGGCGC12450.029.37305615
GTGCGAG37800.028.93099210
GCCAGTA23750.028.0397071
GGCGGGC13550.027.24261313
ATGTGTG44900.026.1998216
ACATGTG43750.026.0999834
TGCGAGT41800.026.07994511
TGACATG44200.025.6781622
GACATGT45800.025.0823973
GCGAGTC46050.023.82283212
TCGGGCG17750.022.1564187
ATCGGGC18000.021.6568986
TAGCCCG17400.021.41292434
GGCGCAT17800.020.93281417
ACCCGTA52850.020.88846232
CGAGTCA54000.020.63540613
Produced by FastQC (version 0.11.2)