FastQCFastQC Report
Wed 13 Sep 2017
HKWY5BGX3_n01_col_10n_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKWY5BGX3_n01_col_10n_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7785816
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG99920.12833593806994667No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTACGTGGAGGCATCCCTTCAC86930.11165175236609753No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGG85300.10955820173505257No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG24900.032.419098
GCCAGTA19300.028.7826651
TGTGCGA30400.026.5537959
TGTGTGC32350.025.1664567
GTGCGAG32500.024.83817110
TGCGAGT33050.024.42482811
CGCGCGT13100.023.96942765
CTGACAT36050.023.9274921
GCGCGCG14600.021.5069564
ACATGTG38850.021.488494
GACATGT40900.021.2548873
CGGGCGC8000.021.13091715
CGTGCGG18350.020.87048769
TGCCCGC24700.020.67200316
ATGTGTG41000.020.614076
TGACATG41500.020.2828432
ATGCCCG25700.019.8673915
GCGAGTC39950.019.86086712
GTAGCGC16200.019.59492561
GCGTGCG16700.018.8021268
Produced by FastQC (version 0.11.2)