FastQCFastQC Report
Wed 13 Sep 2017
HKWY5BGX3_n01_col_05n_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKWY5BGX3_n01_col_05n_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13126809
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG182430.13897513097051994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG38150.032.825128
TGTGTGC49800.025.4925167
TGTGCGA49550.025.4819879
GTGCGAG50650.024.72424710
CGGGCGC15350.024.49818415
CTGACAT55850.023.9082741
TGCGAGT53800.023.2126911
GGCGGGC17600.021.75827213
GCCAGTA36650.021.5586661
ACATGTG63250.021.1621824
GACATGT66900.020.7810843
ATGTGTG62200.020.7430276
GCGAGTC62200.020.13339812
TGACATG68900.019.1764772
ACCCGTA67900.018.18922232
ACGGGTG69100.018.12421420
TGCCCGC39950.017.8762316
TAGCCCG21350.017.61311134
CGGGTGA71550.017.55176221
GGCCGAA44050.017.38679326
Produced by FastQC (version 0.11.2)