FastQCFastQC Report
Wed 13 Sep 2017
HKWY5BGX3_n01_col_05n_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKWY5BGX3_n01_col_05n_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17171103
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG209290.12188500645532206No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTACGTGGAGGCATCCCTTCAC200210.1165970526179943No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGG178290.10383141956576698No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAATTCAGACTGTGAAACTG176410.10273655687698105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG47550.031.41418
TGTGCGA62450.024.3056669
TGTGTGC63000.024.2029197
GCCAGTA50800.024.1803931
GTGCGAG64300.023.2308710
CTGACAT75500.022.119511
CGGGCGC19100.021.67544615
TGCGAGT69650.021.44650711
TGCCCGC58800.020.82961816
ACATGTG78300.020.1782474
CGCGCGT30200.019.9943965
ATGCCCG61600.019.93835815
GGCGGGC20800.019.9034313
CCCTACT65000.019.3185277
ATGTGTG84350.019.1809446
GCGATTT95050.019.167211
GCGCGCG31800.018.9884464
GACATGT87100.018.9316923
TACGTGG82050.018.67056555
GCGAGTC81050.018.51520712
Produced by FastQC (version 0.11.2)