FastQCFastQC Report
Wed 13 Sep 2017
HKWY5BGX3_n01_col_01n_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKWY5BGX3_n01_col_01n_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6463549
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG71240.11021808607005222No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG16700.031.6064918
TGTGTGC22000.024.4626357
TGTGCGA22700.023.404339
GCCAGTA19200.023.1819671
GTGCGAG23100.022.55119910
CTGACAT26850.021.5887151
TGCGAGT24350.021.5352211
CGGGCGC6950.021.3451115
ACATGTG27900.019.7835244
ATGTGTG28050.019.5551996
GGCGGGC7600.019.51893813
GCGAGTC27850.018.82867212
TGCCCGC16150.018.7987216
GCCGCGC5900.018.7135660
ACCCGTA28300.018.28575332
GACATGT31700.018.1739143
TGACATG30750.017.5013162
ACGGGTG30550.017.27886220
ATGCCCG17300.017.15011815
CCGCGCC6450.017.1178361
Produced by FastQC (version 0.11.2)