FastQCFastQC Report
Wed 18 Oct 2017
HKWKVBGX3_n02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKWKVBGX3_n02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300747626
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17338510.5765136114490892No Hit
TCCTTGGGGTAATGCACCTGGTGCAGCAGCTAATCGGGTGGCTTTAGAAG6576860.2186836879636749No Hit
GTGTTATACCGGTGGCTTCAGGGGGTATTCATGTTTGGCATATGCCAGCT6441930.2141972020088365No Hit
GTCATATATAGCCTCGTTTAATTGTTCATCTCTGATTCGATGATGTCTCC4680930.15564312384630427No Hit
CTTTAGGACATCCTTGGGGTAATGCACCTGGTGCAGCAGCTAATCGGGTG4593860.15274800539905176No Hit
GTGTACAAGCTCGTAACGAAGGGCGCGATCTTGCTCGTGAAGGTAATGAA3241820.10779203956210115No Hit
TTAGAAGCCTGTGTACAAGCTCGTAACGAAGGGCGCGATCTTGCTCGTGA3169490.10538703304677126No Hit
TGCCAGCTCTGACCGAAATCTTTGGAGATGATTCTGTATTGCAATTTGGT3065200.10191934150130248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG2604150.022.0091135
GTTATAC2590450.021.8540123
ATACCGG2563350.021.637256
TTATACC2594000.021.4643964
TACCGGT2679700.020.5624317
CCGGTGG2663900.020.1904539
GGACCAT1071300.019.9459556
TCAGGGG2801550.019.52845218
GGCTTCA2763700.019.51997614
TGTTATA3052350.019.4881742
TGGCTTC2752800.019.48525413
ACCGGTG2841950.019.2673878
TTCAGGG2848700.018.9192817
GGGGTAT2601300.018.91631722
GGTATTC2610700.018.80375924
GGGGGTA2863050.018.66029221
GGGTATT2665300.018.45492423
CGGTGGC2945500.018.34237310
CAGGGGG3005750.018.22741319
GCTTCAG3031400.017.98165115
Produced by FastQC (version 0.11.2)