FastQCFastQC Report
Wed 18 Oct 2017
HKWKVBGX3_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKWKVBGX3_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300747626
Sequences flagged as poor quality0
Sequence length20
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG4682270.15568767947647907No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACAA4528450.013.9179981
GCCGAAC9735450.013.8769345
GAGGAAC8124250.013.8668651
AGGAACA8128750.013.8665792
TTCCACC3868650.013.8636422
TTCGACC3903750.013.8560771
CCGAACA9748700.013.8509866
CGCATCT4881200.013.8483775
TCGACCA3915950.013.8365252
CCTCTCT2253600.013.8283294
CGCATCC3188450.013.82595
TACTCCA3465050.013.8214993
CCTTCTT2769450.013.8209031
CCACACT3048400.013.8207972
TGCCGAA9789450.013.81790354
CACGCTA5655900.013.8147644
CTCCTAC2482050.013.8146124
CTACTCC3470000.013.81370452
CGTCTCT4366350.013.8107362
GGCATCT3315150.013.8088446
Produced by FastQC (version 0.11.2)