FastQCFastQC Report
Tue 10 Oct 2017
HKWJFBGX3_n02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKWJFBGX3_n02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278811800
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12741730.4570011025358324No Hit
GTGTTATACCGGTGGCTTCAGGGGGTATTCATGTTTGGCATATGCCAGCT7839890.2811893183860941No Hit
TCCTTGGGGTAATGCACCTGGTGCAGCAGCTAATCGGGTGGCTTTAGAAG3170010.11369712472714569No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG2497600.020.5682895
GTTATAC2408900.020.5673983
TTATACC2496550.020.0188624
TACCGGT2513750.019.7442347
GGACCAT1393150.019.4519946
ATACCGG2728400.019.3922776
TGTTATA2640750.019.228832
GGGGTAT2428050.018.8996322
GGGGGTA2521300.018.84528521
GGTATTC2475400.018.66622524
TCAGGGG2632200.018.3466118
GGCTTCA2630450.018.277614
GGGTATT2533550.018.15705323
GTGTTAT2847650.018.0616911
TGGCATA2430400.018.03769336
GGTATCA471600.017.8861141
TTCAGGG2695550.017.87078317
ACCGGTG2762650.017.8666258
TGGCTTC2676800.017.85493313
TGGACCA1646400.017.6836265
Produced by FastQC (version 0.11.2)