FastQCFastQC Report
Tue 10 Oct 2017
HKWJFBGX3_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKWJFBGX3_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278811800
Sequences flagged as poor quality0
Sequence length20
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG3919150.1405661453353122No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCTAC4727150.013.87634854
TAGACCT5399750.013.8581014
TACACCT3475500.013.8559741
TCTCCTA4740800.013.8472473
TCAGAGC4763150.013.84614851
ACACCTT3476700.013.8456232
CCTACGT4723850.013.8435136
TCCTACG4730450.013.839025
CCTCTCT2214800.013.8382164
AGTCTCC4713100.013.8379231
AGACCTT5392450.013.8346975
AATCCTT4352450.013.8326282
GTCTCCT4753500.013.8230452
CCTATAG3469000.013.8224782
TCCTTAC4344550.013.8171774
CACCTTC3479750.013.8165693
CCTTACG4338900.013.8158325
ACACCAT3652100.013.8140913
TTAGACC5418750.013.8134783
CCTTCGT3473500.013.813425
Produced by FastQC (version 0.11.2)