FastQCFastQC Report
Fri 27 Oct 2017
HKW5JBGX3_n01_mb64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKW5JBGX3_n01_mb64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25165394
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG262478810.43014863983453TruSeq Adapter, Index 14 (98% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC2984500.065.1003851
CCGTCTT2985350.065.08427450
TGCCGTC2990200.065.04098548
GCCGTCT2988500.065.0202849
ATGCCGT2998100.064.9020747
TATGCCG3002100.064.85490446
CTCGTAT2992850.064.7319942
CGTATGC3010800.064.7316344
TCTCGTA2980500.064.713841
TCGTATG3011550.064.68008443
ATCTCGT2981100.064.5413440
CATCTCG2972700.064.414339
ACACGTC3104850.064.19775413
GTATGCC3034750.064.1936445
CGTCTGA3092700.064.1932216
ACGTCTG3100650.064.08523615
CACGTCT3106400.064.0465614
GCTTGAA3049950.063.82131259
CACACGT3129500.063.73054512
GCACACG3133550.063.7150711
Produced by FastQC (version 0.11.2)