FastQCFastQC Report
Fri 27 Oct 2017
HKW5JBGX3_n01_mb63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKW5JBGX3_n01_mb63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24893769
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG18241727.327825690035125TruSeq Adapter, Index 13 (98% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT2140800.063.7680150
ATGCCGT2143750.063.72028447
CTCGTAT2123350.063.71342
AATCTCG2112650.063.71281439
TCTCGTA2121950.063.70965241
TGCCGTC2143200.063.70152348
GCCGTCT2143400.063.66729749
CGTCTTC2144750.063.64740451
CGTATGC2149500.063.64444444
TCGTATG2150700.063.64071343
TATGCCG2149050.063.59738546
CGTCTGA2228500.063.53162416
ATCTCGT2130650.063.40537340
ACGTCTG2242050.063.2326615
ACACGTC2253200.063.17323713
CACGTCT2255200.063.00147614
GTATGCC2180600.062.69178845
CACACGT2278550.062.48339512
GCACACG2284900.062.3897611
GCTTGAA2201600.062.07040459
Produced by FastQC (version 0.11.2)