FastQCFastQC Report
Fri 27 Oct 2017
HKW5JBGX3_n01_mb60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKW5JBGX3_n01_mb60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23867969
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG2332180.9771170726759365TruSeq Adapter, Index 11 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA282800.062.1741241
CTCGTAT284500.061.89214742
TCGTATG286350.061.7485343
CGTATGC288200.061.42552644
ATGCCGT290200.060.97316747
TGCCGTC291550.060.6330648
TATGCCG292050.060.60751746
CCGTCTT292250.060.46916250
GCCGTCT292500.060.415249
CGGCTAC292250.059.82805633
ATCTCGT293400.059.7854240
ACGGCTA295850.059.6130532
CATCTCG295450.059.17024239
CGTCTTC298750.059.1540151
TCACGGC309000.057.2320430
GTCACGG315250.056.33809729
CACGGCT315550.056.03288331
AGTCACG321400.055.30299828
GTATGCC321850.055.13374745
CGTCTGA353900.051.2001516
Produced by FastQC (version 0.11.2)