FastQCFastQC Report
Fri 27 Oct 2017
HKW5JBGX3_n01_mb59.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKW5JBGX3_n01_mb59.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25635249
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG4081071.5919759546708518TruSeq Adapter, Index 10 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT476200.063.4932442
TCGTATG478100.063.43710743
TCTCGTA477050.063.31805441
ATGCCGT480350.063.1202247
CGTATGC481200.063.0609244
TATGCCG483100.062.79417446
TATCTCG480700.062.7686439
GCCGTCT483500.062.57089649
TGCCGTC484900.062.45173348
CCGTCTT486250.062.2965550
ATCTCGT486700.062.03317340
CGTCTTC492000.061.4925451
GTATGCC514400.059.0790145
GCTTATC515200.058.42862336
ACTAGCT547450.055.74912332
TAGCTTA547700.055.34569534
GCTTGAA553100.054.9208359
CGTCTGA566100.054.741816
CACTAGC559700.054.70715331
AGCTTAT552950.054.69545735
Produced by FastQC (version 0.11.2)