FastQCFastQC Report
Fri 27 Oct 2017
HKW5JBGX3_n01_mb58.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKW5JBGX3_n01_mb58.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25668703
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG387159415.082935822663107TruSeq Adapter, Index 9 (100% over 63bp)
GATCGGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG291920.11372604217673173TruSeq Adapter, Index 9 (100% over 59bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG4330400.064.9819939
CTCGTAT4370800.064.8929842
TCTCGTA4362350.064.8758341
TGCCGTC4394600.064.8611648
ATGCCGT4397900.064.8581147
CCGTCTT4392100.064.84408650
GCCGTCT4393100.064.8432749
TCGTATG4406250.064.8353843
CGTATGC4405400.064.8343344
TATGCCG4404100.064.7975646
CGATCAG4383800.064.79497533
CGTCTTC4397600.064.7859751
ATCTCGT4349250.064.78548440
ACGATCA4402950.064.74348432
CGTCTGA4477650.064.70663516
ACACGTC4502500.064.6969713
CACGATC4410500.064.6462331
GTCACGA4414650.064.63531529
ACGTCTG4489600.064.59740415
TCACGAT4419300.064.5686230
Produced by FastQC (version 0.11.2)