FastQCFastQC Report
Fri 27 Oct 2017
HKW5JBGX3_n01_mb57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKW5JBGX3_n01_mb57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38923563
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG37474889.627813363334697TruSeq Adapter, Index 8 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG589770.1515200445550167TruSeq Adapter, Index 8 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG4223800.065.690239
CGTCTGA4486150.065.2324316
ACGTCTG4503400.065.01269515
ACACGTC4528250.064.8601613
CACGTCT4528950.064.7475714
TCTCGTA4297800.064.73641
CTCGTAT4304100.064.70946542
ATGCCGT4338600.064.64664547
TCGTATG4337700.064.6439343
CGTATGC4344800.064.6147144
TGCCGTC4334550.064.6003148
GCCGTCT4325950.064.56441549
CCGTCTT4331000.064.54681450
TATGCCG4348700.064.54629546
ATCTCGT4309000.064.54487640
CGTCTTC4333450.064.418851
TTGAATC4316600.064.3023936
GAATCTC4322900.064.155938
GAACTCC4536050.064.15262621
CACACGT4582600.064.1157612
Produced by FastQC (version 0.11.2)