FastQCFastQC Report
Fri 27 Oct 2017
HKW5JBGX3_n01_mb56.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKW5JBGX3_n01_mb56.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26535900
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG4776121.7998711179948674TruSeq Adapter, Index 3 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT558400.063.52422342
TCGTATG561100.063.52209543
TCTCGTA557150.063.39785441
ATGCCGT562300.063.35361547
CGTATGC563200.063.31801244
TATGCCG566550.062.91123246
TGCCGTC566750.062.852148
GCCGTCT568500.062.66849
CCGTCTT571800.062.3013550
ATCTCGT567100.062.2417640
CATCTCG569750.061.74326339
CGTCTTC578200.061.61267551
GTATGCC596100.059.90699445
CGTCTGA623500.058.59805716
ACGTCTG632150.057.84501315
ACACGTC634400.057.81852713
CACGTCT645200.056.7974514
GCTTGAA635950.056.17696459
CTTAGGC640000.055.57188833
TAGGCAT637650.055.3704635
Produced by FastQC (version 0.11.2)