FastQCFastQC Report
Fri 27 Oct 2017
HKW5JBGX3_n01_mb55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKW5JBGX3_n01_mb55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25764918
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG2526040.9804184123543493TruSeq Adapter, Index 1 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG310900.062.0670743
CTCGTAT308950.061.943242
TCTCGTA309150.061.71929641
CGTATGC314750.061.35481344
TATGCCG314350.061.31667346
ATGCCGT318100.060.6610347
ACGATCT314800.059.9012737
ATCTCGT318850.059.48268540
CACGATC318150.059.18368536
CGATCTC320250.058.9691838
GATCTCG323000.058.650639
TCACGAT324650.058.40212235
TGCCGTC331100.058.27181648
CCGTCTT330650.058.2510550
ATCACGA328450.057.95747434
CGTCTTC341200.056.5313851
GCCGTCT341700.056.43614649
GTATGCC346000.055.82562345
CATCACG345950.055.43445233
GCTTGAA386100.050.11934759
Produced by FastQC (version 0.11.2)