FastQCFastQC Report
Fri 27 Oct 2017
HKW5JBGX3_n01_mb54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKW5JBGX3_n01_mb54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26517668
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG22581708.515718652183141TruSeq Adapter, Index 12 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG2512050.065.54114539
TCTCGTA2519300.065.49814641
CTCGTAT2522600.065.4733442
ATGCCGT2535400.065.4692347
CGTATGC2538900.065.4491644
TCGTATG2539450.065.4402943
TATGCCG2539800.065.3829846
GCCGTCT2535800.065.35187549
TGCCGTC2539800.065.2939248
CCGTCTT2537950.065.2935450
ATCTCGT2528350.065.22687540
CGTCTTC2545450.065.11251
CGTCTGA2597250.064.9922316
ACACGTC2611450.064.8207413
ACGTCTG2608050.064.7668715
GTATGCC2573050.064.5739245
CACGTCT2620550.064.5429914
GTAATCT2546500.064.4774437
TAATCTC2568500.064.0041438
TGTAATC2565100.064.0001336
Produced by FastQC (version 0.11.2)