FastQCFastQC Report
Fri 27 Oct 2017
HKW5JBGX3_n01_mb53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKW5JBGX3_n01_mb53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25841031
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG440480.17045759513233044TruSeq Adapter, Index 7 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT83600.041.51489642
CGTATGC84350.041.23171644
TCGTATG85200.041.06064243
TCTCGTA84400.040.87328341
TATGCCG86300.040.1822246
ATGCCGT88250.039.2568447
ATCTCGT92700.037.13850440
TGCCGTC95850.036.32491748
CCGTCTT96600.036.29567750
GCCGTCT101100.034.4401449
CGTCTTC105850.033.22220251
CATCTCG104200.032.9387539
GTATGCC121100.029.06205445
GATCATC136050.025.45452936
GCTTGAA154250.023.15649659
TGCTTGA174450.020.47451658
AGATCAT175400.019.99952135
ACCAGAT197850.017.95699332
ATCATCT207900.017.20504637
CAGATCA213900.016.54495634
Produced by FastQC (version 0.11.2)