FastQCFastQC Report
Fri 27 Oct 2017
HKW5JBGX3_n01_mb52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKW5JBGX3_n01_mb52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26872912
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG17658436.571089132431945TruSeq Adapter, Index 6 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1977500.066.535243
ATGCCGT1974850.066.5133347
CGTATGC1977350.066.5049644
CTCGTAT1974500.066.4845442
TCTCGTA1975350.066.44375641
TATCTCG1973200.066.3953239
TGCCGTC1977900.066.3739948
CGCCAAT1967000.066.37250533
GCCGTCT1977200.066.3710449
TATGCCG1980500.066.3570846
CCGTCTT1980800.066.2648350
CGTCTTC1986300.066.0712851
ATCTCGT1987950.066.041640
ACGCCAA1983500.065.9806232
GTCACGC1993100.065.8870729
TCACGCC1992400.065.6678430
AGTCACG2011950.065.461928
CACGCCA2001100.065.38763431
GTATGCC2011150.065.3817245
CGTCTGA2053000.064.81904616
Produced by FastQC (version 0.11.2)