FastQCFastQC Report
Fri 27 Oct 2017
HKW5JBGX3_n01_mb51.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKW5JBGX3_n01_mb51.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24130015
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG708620.29366745109773035TruSeq Adapter, Index 5 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG97100.055.1789643
CTCGTAT97300.054.74494642
TATGCCG99900.053.7771246
TCTCGTA99400.053.20382341
CGTATGC102100.052.54662344
ATGCCGT103000.052.12691547
ATCTCGT106850.049.36356740
TGCCGTC111550.048.25706548
GATCTCG111150.047.42106239
CCGTCTT116600.046.3180750
GCCGTCT119000.045.2373449
CGTCTTC126450.042.8195851
GTATGCC131700.041.18391445
AGTGATC163250.032.53913536
GCTTGAA172450.031.61901959
GTGATCT175650.030.34022737
TGCTTGA189350.028.81438458
TGATCTC189250.028.32452238
GTCACAC237100.023.11682129
GTCTTCT248200.022.32975252
Produced by FastQC (version 0.11.2)