FastQCFastQC Report
Fri 27 Oct 2017
HKW5JBGX3_n01_mb50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKW5JBGX3_n01_mb50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33725852
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG31211709.254532694978321TruSeq Adapter, Index 4 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG3473650.065.9382539
TCTCGTA3489350.065.90587641
ATGCCGT3531200.065.9054647
CTCGTAT3497100.065.8884242
TCGTATG3538050.065.8805443
CGTATGC3538950.065.8658344
GCCGTCT3532550.065.8276249
TATGCCG3538350.065.8179246
TGCCGTC3534450.065.81058548
CCGTCTT3534200.065.7985450
CGTCTTC3542950.065.64101451
ATCTCGT3499500.065.6367640
GTATGCC3579800.065.07326545
ACCAATC3503100.064.8098136
GCTTGAA3612150.064.47874559
GACCAAT3557050.064.4701535
CGTCTGA3669750.064.359916
CCAATCT3546100.064.2536837
CAATCTC3556750.064.2038338
ACTGACC3604300.064.1824632
Produced by FastQC (version 0.11.2)