FastQCFastQC Report
Fri 27 Oct 2017
HKW5JBGX3_n01_mb49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKW5JBGX3_n01_mb49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33266335
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG399070.11996211785879028TruSeq Adapter, Index 2 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG70050.043.09483343
CTCGTAT70200.042.75618442
CGTATGC72800.041.56390444
TATGCCG74600.040.74914646
TCTCGTA73800.040.6203541
ATGCCGT76400.039.6556847
TATCTCG78100.038.29267539
ATCTCGT86100.035.0567840
CGATGTA87200.034.05708334
TGCCGTC90050.033.4926148
CCGTCTT93100.032.69450850
CCGATGT97400.030.63218533
GCCGTCT100050.030.25008849
ACCGATG101450.029.6135332
CACCGAT104850.028.65311631
CGTCTTC107150.028.3434251
GTATGCC119550.025.68680445
TCACCGA126350.024.02312930
GTCACCG140950.021.5837529
GTATCTC148700.020.5521538
Produced by FastQC (version 0.11.2)