FastQCFastQC Report
Fri 15 Dec 2023
HKVTJDRX3_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVTJDRX3_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493293945
Sequences flagged as poor quality0
Sequence length8
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGAACT354909117.194678012923918No Hit
CACCTACA317722216.440829311213216No Hit
TTCACGCA278264835.640953691414153No Hit
GTTAACCG185906643.7686787337314667No Hit
TACTCCTT170949313.465465403188762ABI Solid3 GAPDH Reverse Primer (100% over 8bp)
TCTACTCT168872753.4233696097769863No Hit
GGTCTATG164709733.338977331254289No Hit
CTCCTTAC147386072.9877940220814994No Hit
AGAGGATA128223472.5993319257141905No Hit
TATGCAGT120328642.439288809839334No Hit
TCAGGCAA113586222.3026072213393984No Hit
ATAGAGAG103344492.094988009633891ABI Solid3 Adapter B (100% over 8bp)
TTCTCTGC94146621.9085298117737892No Hit
GGACGTAT93963711.9048218805929191No Hit
AGGCTTAG89797651.8203679755282625No Hit
TGTTACGG87712391.7780958166839043No Hit
GAGGCTAT86396241.7514149702364579No Hit
CCAATGCT86381511.7511163653143968No Hit
TTCCGTCT80993051.6418821033775308TruSeq Adapter, Index 14 (100% over 8bp)
TCTCAGTG78342461.58814963763644No Hit
GGCTAACA75838721.5373940987660004No Hit
CTGTTGGA72949991.478834085425476No Hit
GGGGGGGG65982161.3375830104705622No Hit
TTCCACGA65646981.3307882787817313No Hit
GTTCCTAG50458181.022882614137905No Hit
TTATGGCC47580170.9645399154453437No Hit
CTCTAGCT46855560.9498507021001443No Hit
GTCGGTAA46664260.9459726897722209No Hit
TGGTTCAC44353170.8991225302795882No Hit
TAGCACAG43641370.8846929998299493No Hit
GCGATCTA42140080.8542590158896031No Hit
CTACACTA41232250.8358555870780047No Hit
CCTATCAC41088330.8329380568415451No Hit
GCTCACAA40654970.8241530311100819No Hit
TCTCGAAC40522160.8214607215582181No Hit
CGAGAGTT40069670.8122878945939627TruSeq Adapter, Index 13 (100% over 8bp)
TAGCGACG38410080.7786448706561765No Hit
CACGTCGT36845510.7469280815924063No Hit
AGCTCTAG36371410.7373171791111282No Hit
CGTACGCA36349930.7368817389396498No Hit
GGTACCTT35332210.7162506322675418No Hit
TCCTCAAG35144660.7124486395226278No Hit
TTGTGCGT34216800.6936391647783149No Hit
TGCAACCT33922080.6876646337104341No Hit
TCCAGAGA30482840.6179447428652302No Hit
GCATCCAT29997940.6081149039848848No Hit
TTCAGCAC29372840.595442946294425No Hit
TCAGTGTC27521240.5579075169876655No Hit
GAAGTCAG27519530.5578728520578131No Hit
GTTGTGAC22801560.4622306888441535No Hit
ACTAGAGC21867790.44330140723701766No Hit
GTTCGACA20347550.41248327100386367No Hit
GTGTATAT20329450.41211634981653783No Hit
GAAGGACT17150270.34766836637331927No Hit
GACTTCGA12026160.24379297824140125No Hit
GAGCTAAC11267880.2284212104002209No Hit
GACCATTG10455770.2119582067848005No Hit
CTAGAGAG10120250.20515658265377654No Hit
CGAGGATA9648920.1956018333044814No Hit
TGAGGATA8302430.1683059377507664No Hit
AGATCTCG7964250.16145039039552775Illumina Single End PCR Primer 1 (100% over 8bp)
CCACGCGT6691430.13564792488989502No Hit
CGTAAGGA6685920.13553622678259308No Hit
CATGGCCA6128270.12423160799186375No Hit
TTAGAGAG5045230.10227634154317462No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)