FastQCFastQC Report
Fri 15 Dec 2023
HKVTJDRX3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVTJDRX3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493293945
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGACAC187404303.79903913071546No Hit
AGCACCTC175795413.5637050035146896No Hit
TTCACGCA168893783.423795927598503No Hit
TGGCTTCA162090403.285878564757165No Hit
AGCCATGC157713443.1971493183440556No Hit
ATCATTCC140077042.8396261786671637No Hit
ACAGATTC136227742.7615935970995955No Hit
TCTTCACA117322212.378342795186752No Hit
TCCGTCTA112925352.2892101381864722No Hit
AATCCGTC110974612.2496649538238302No Hit
TAGCGCTC110109902.2321356488573967No Hit
CGGAGCCT107360722.1764045775992646No Hit
ACACGACC101774072.063152630020626No Hit
AAGAGGCA100384982.0349931520039233No Hit
GCTACGCT97529421.9771055572149785No Hit
GGGGGGGG96510571.9564515433085234No Hit
GCGTAGTA94825911.9223003031184582No Hit
GGACTCCT94710201.9199546428651177No Hit
TATCAGCA91647771.857873402439594No Hit
ACTCGCTA90454931.8336922826003874No Hit
ATCTCAGG89460191.8135270239329613No Hit
AGGCAGAA88978991.8037721910411852No Hit
CGAGGCTG88958731.8033614825740463No Hit
GTAGAGGA87699771.7778399854472164No Hit
GCTCATGA87440671.7725875390584815No Hit
GGAGCTAC87143851.7665704370241155No Hit
TACGCTGC84812161.7193026766221506No Hit
CTCTCTAC84705061.7171315573313999No Hit
ACTGAGCG84183031.7065490232198166No Hit
ATGCGCAG82903251.6806054653681184No Hit
CGTACTAG81187301.645819917777422No Hit
CAGAGAGG79312691.607818032309316No Hit
TAGGCATG79019281.6018700574157667No Hit
TCCTGAGC67094351.360129202477845No Hit
TAAGGCGA57658041.168837375451669No Hit
CATTACGG50386791.0214354039962927No Hit
ACAGTGGT49308880.9995841323371606No Hit
CACTAGCT45611210.9246253772687195Illumina PCR Primer Index 10 (100% over 8bp)
GGTTGCAA43756230.8870214289778076No Hit
GCATAAGG40705390.8251751397435053No Hit
GAACCACA38278560.775978711840868No Hit
TAGTCTCC37453450.7592521736710147No Hit
GATTCGGA37080880.7516994760598572No Hit
CCTAAGAC34853020.7065365458722588No Hit
GCGACTAA34508650.6995555155253325No Hit
AACCTCCA34326310.6958591393210797No Hit
TGAGTACG33369430.6764613743637173No Hit
ACCGGTAA31800920.6446647140580652No Hit
ACTACGAC29208070.5921027471764325No Hit
CGTGTACA27435640.5561722433061691No Hit
CATCTTCG26338010.533921209999851No Hit
GTCTGCTA24231380.4912158408917831No Hit
GACATAGT23491390.4762148458968009No Hit
CGAGCGAC23208440.47047891496012584No Hit
ACTCACTG22832230.46285242767372703No Hit
CGAGAGTT20423020.4140131904517903TruSeq Adapter, Index 13 (100% over 8bp)
ACGCTACT19236500.3899601889498157No Hit
CGATCAGT19177940.3887730671415397No Hit
CTGCGTAG18122150.36737020966271944No Hit
GTCTATGA15612290.3164906068328084No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)