FastQCFastQC Report
Fri 15 Dec 2023
HKVTJDRX3_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVTJDRX3_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493293945
Sequences flagged as poor quality0
Sequence length301
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG5375097410.89633767955534No Hit
CTGTAAAGATAAATCGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG279748095.67102217319939No Hit
GTAACCGGATTAGATACCCGGTTCAGAAGGACTGTCTCTTATACACATCT211711444.2917907699029225No Hit
GTAACAGGATTAGATACCCGGTTCAGAAGGACTGTCTCTTATACACATCT163574403.3159620477401153No Hit
GTAACCGGATTAGAAACCCGGTTCAGAAGGACTGTCTCTTATACACATCT101452602.0566358259272772No Hit
GTAACAGGATTAGAAACCCGGTTCAGAAGGACTGTCTCTTATACACATCT88492991.7939200530831572No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGAATGGAGCTGGGATTGACTCG69747761.413918834945359No Hit
CTGTAAAGATAAATCGGTCATCTAAAGAGAATGGAGCTGGGATTGACTCG58494741.1857988648127438No Hit
GTAACCGGATTAGATACCCGCTTCAGAAGGACTGTCTCTTATACACATCT40423130.819453196410104No Hit
CTGTAAAGATAAATCGGTGATCTAAAGAGTATGGAGCTGGGATTGACTCG39652930.8038397876543973No Hit
CTGTAAAGATAAATGGGTCAACTAAAGAGTATGGAGCTGGGATTGACTCG34311150.6955518174868334No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACGCG33458380.6782645588727021No Hit
GTAACCGGATTAGAAACCCGCTTCAGAAGGACTGTCTCTTATACACATCT33344960.6759653212447195No Hit
CTGTAAAGATAAATCGGTCAACTAAAGAGTATGGAGCTGGGATTGACTCG31914240.6469619244971677No Hit
CTGTAAAGATAAATGGGTGATCTAAAGAGTATGGAGCTGGGATTGACTCG31739950.6434287369977751No Hit
CTGTAAAGATAAATGGTTCATCTAAAGAGTATGGAGCTGGGATTGACTCG31258060.6336599165027255No Hit
GTAACAGGATTAGATACCCGCTTCAGAAGGACTGTCTCTTATACACATCT30969860.627817558149837No Hit
GTAACCGGATTAGAAAACCGCTTCAGAAGGACTGTCTCTTATACACATCT29972850.6076062822948293No Hit
CTGTAAAGATAAATCGTTCATCTAAAGAGTATGGAGCTGGGATTGACTCG29024070.5883727196367675No Hit
GTAACAGGATTAGAAACCCGCTTCAGAAGGACTGTCTCTTATACACATCT27730920.5621581266317793No Hit
GTAACAGGATTAGAAAACCGCTTCAGAAGGACTGTCTCTTATACACATCT26704260.5413457892737767No Hit
CTGTAAAGATAAATCGTTCATCTAAAGAGAATGGAGCTGGGATTGACTCG25803480.5230852772782362No Hit
GGCTTGGTCATTTAGAGGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTG25632860.519626487610749No Hit
CTGTAAAGATAAATCGGTCAACTAAAGAGAATGGAGCTGGGATTGACTCG25542790.5178005985863053No Hit
CTGTAAAGATAAATGGTTCATCTAAAGAGAATGGAGCTGGGATTGACTCG25334230.5135726934576503No Hit
GTAACCGGATTAGATAACCGCTTCAGAAGGACTGTCTCTTATACACATCT23983270.486186182560988No Hit
CTGTAAAGATAAATGGGTCAACTAAAGAGAATGGAGCTGGGATTGACTCG23264750.47162042501859613No Hit
GTAACCGGATTAGAAAACCGCTTCAGAAGCACTGTCTCTTATACACATCT21550720.43687379945440036No Hit
GTAACAGGATTAGAAAACCGCTTCAGAAGCACTGTCTCTTATACACATCT20573340.41706046077658626No Hit
GTAACCGGATTAGAAACCCGCTTCAGAAGCACTGTCTCTTATACACATCT20386820.4132793480771389No Hit
GTAACAGGATTAGATAACCGCTTCAGAAGGACTGTCTCTTATACACATCT19602450.3973786866571006No Hit
GTAACCGGATTAGATACCCTGTTCAGAAGGACTGTCTCTTATACACATCT19079090.3867691909334099No Hit
GGCTTGGTCATTTAGAGGTAGTAAAAGTCGTAACAAGGTTTCCGTAGGTG19053010.38624050007343996No Hit
GTAACCGGATTAGATACCCGCTTCAGAAGCACTGTCTCTTATACACATCT18866080.38245107589958355No Hit
GTAACAGGATTAGAAACCCGCTTCAGAAGCACTGTCTCTTATACACATCT18382630.37265063125800174No Hit
CTGTAAAGATAAATCGTTCAACTAAAGAGAATGGAGCTGGGATTGACTCG17094220.3465321270059376No Hit
GTAACCGGATTAGATACCCGGTTCAGAAGCACTGTCTCTTATACACATCT15864570.32160479893990995No Hit
GTAACAGGATTAGATACCCGCTTCAGAAGCACTGTCTCTTATACACATCT15717510.31862361497261027No Hit
CTGTAAAGATAAATCGTTCAACTAAAGAGTATGGAGCTGGGATTGACTCG15493250.31407744118975556No Hit
GTAACAGGATTAGATACCCTGTTCAGAAGGACTGTCTCTTATACACATCT14022710.2842668178300871No Hit
GTAACAGGATTAGATACCCGGTTCAGAAGCACTGTCTCTTATACACATCT12669020.25682496467699395No Hit
GTAACCGGATTAGAAACCCGGTTCAGAAGCACTGTCTCTTATACACATCT12396460.2512996586649771No Hit
GTAACCGGATTAGATAACCGCTTCAGAAGCACTGTCTCTTATACACATCT11959850.2424487492949057No Hit
CTGTAAAGATAAATGGTTCAACTAAAGAGTATGGAGCTGGGATTGACTCG11530820.23375150084195742No Hit
CGGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG11371140.2305144856379699No Hit
CTGTAAAGATAAATCGGTCATCTAAAGAGTATGGAGCTGGGATTGACGCG11279800.22866285131474703No Hit
CTGTAAAGATAAATGGTTCAACTAAAGAGAATGGAGCTGGGATTGACTCG11114950.2253210304456504No Hit
GTAACAGGATTAGAAACCCGGTTCAGAAGCACTGTCTCTTATACACATCT10930870.22158938115488122No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGAATGGAGCTGGGATTGACGCG10924000.22145011327880784No Hit
GTAACAGGATTAGATAACCGCTTCAGAAGCACTGTCTCTTATACACATCT10630680.21550396285525053No Hit
CTGTAAAGATAAATGGGTCATCTAATCCGGTTACCTGTCTCTTATACACA10055500.2038439778538129No Hit
GTAACCGGATTAGATAACCGGTTCAGAAGGACTGTCTCTTATACACATCT9897910.20064933089742262No Hit
GTAACCGGATTAGAAAACCGGTTCAGAAGGACTGTCTCTTATACACATCT9614620.19490650751855468No Hit
GTAACCGGATTAGAAACCCTGTTCAGAAGGACTGTCTCTTATACACATCT9482100.19222007681444375No Hit
CTGTAAAGATAAATGGGTCATCTAATCCTGTTACCTGTCTCTTATACACA8944050.18131278704424397No Hit
GTAACAGGATTAGAAAACCGGTTCAGAAGGACTGTCTCTTATACACATCT8574860.17382860841723893No Hit
CGGTAAAGATAAATCGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG8537510.17307145337046453No Hit
GGCTTGGTCATTTAGAGGTAGGAGAAGTCGTAACAAGGTTTCCGTAGGTG8527780.17287420789241595No Hit
GTAACAGGATTAGATAACCGGTTCAGAAGGACTGTCTCTTATACACATCT8248970.1672222025753833No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGTCTCG7800980.15814059911073913No Hit
GGCTTGGTCATTTAGAGGAAGGAGAAGTCGTAACAAGGTTTCCGTAGGTG7663470.15535301168150362No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGTAGCTGGGATTGACTCG7395950.14992987598905153No Hit
GTAACAGGATTAGATACACCGGTTCAGAAGGACTGTCTCTTATACACATC7282550.14763104379884492No Hit
GTAACAGGATTAGAAACCCTGTTCAGAAGGACTGTCTCTTATACACATCT7246750.14690531017971445No Hit
GTAACCGGATTAGATACACCGGTTCAGAAGGACTGTCTCTTATACACATC7105720.14404636570189402No Hit
GTAACCGGATTAGAAACCGGGTTCAGAAGGACTGTCTCTTATACACATCT6486140.1314863088376242No Hit
GTAACCGGATTAGATACCGGGTTCAGAAGGACTGTCTCTTATACACATCT6422690.13020005749310384No Hit
CTGTAAAGATAAATCGGTGATCTAAAGAGTATGGAGCTGGGATTGTCTCG6007300.12177931760342205No Hit
GTAACAGGATTAGAAACCGGGTTCAGAAGGACTGTCTCTTATACACATCT5990170.12143206014823475No Hit
GGCTTGGTCATTTACAGGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTG5955330.1207257875423547No Hit
GTAACAGGATTAGATACCGGGTTCAGAAGGACTGTCTCTTATACACATCT5399360.10945522552481361No Hit
GGCTTGGTCATTTACAGGTAGTAAAAGTCGTAACAAGGTTTCCGTAGGTG5285710.10715132536240639No Hit
GTAAAAGGATTAGATACCCGGTTCAGAAGGACTGTCTCTTATACACATCT5247270.10637207395683725No Hit
CTGTAAAGATAAATCGGTCATCTAAAGAGTATGGAGCTGGGATTGTCTCG5163570.10467531686406571No Hit
CTGTAAAGATAAAGCGGTGATCTAAAGAGTATGGAGCTGGGATTGACTCG4981020.10097468356316434No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)