FastQCFastQC Report
Wed 31 Jul 2024
HKVNYDSXC_n01_CG_87.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVNYDSXC_n01_CG_87.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18500256
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA3387081.830828719343127No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG773600.41815637578204323No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAC482770.2609531457294429No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT470250.25418567180908197No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTTGAACATCTCGTAT294690.15928968766702473TruSeq Adapter, Index 11 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)