FastQCFastQC Report
Thu 12 Mar 2020
HKVNYDRXX_l02_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVNYDRXX_l02_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15714813
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1306090.8311202939545002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTG40150.040.134822
ACCGTGT44000.035.3037223
CCGTGTT46850.032.22654
GTTCACT72800.022.0382678
ATTAGGC50900.018.2537885
TATTAGG48400.017.99684
TTCACTA102450.017.6381119
TCACTAG79800.017.2826423
CTTTATT134800.017.185871
TTAGGCA59050.015.6115026
CACTAGC88450.015.4283494
CTAGCAA106750.013.5315026
CTCAGAA184050.013.4524591
ACTAGCA111300.012.8478925
TAGCAAC114750.012.526487
TTATTAG68750.012.4585863
TGTTCAC144550.012.3045167
CAGACTC113900.011.8535573
GGTTGAG71400.011.3862495
ACTCACC164250.011.3134586
Produced by FastQC (version 0.11.5)