FastQCFastQC Report
Thu 12 Mar 2020
HKVNYDRXX_l02_n02_Emily_2_Mar_9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVNYDRXX_l02_n02_Emily_2_Mar_9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20046944
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG267740.13355651614530376No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC253230.1263185052045838No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC239470.11945461612503133No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA201920.10072358160924677No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT79350.035.6683461
AACCGTG85000.032.9225652
TCACTAG149600.022.5344353
ACCGTGT126150.022.470613
CACTAGC150600.021.8552724
TTCACTA168750.020.7934682
ACTAGCA163600.020.2958625
CCGTGTT137900.020.2405244
ATTAGGC114950.019.5514095
TAGCAAC167500.019.304017
CTAGCAA171150.018.9345916
AACTCAC224850.018.6363265
CGTGTTC155700.018.5784575
GTTCACT195950.018.2215421
TATTAGG121750.018.1616864
ACTCACC231600.017.7175316
AGACTCA184550.016.1062764
GAACTCA283250.015.56176954
AGAACTC293100.014.7914483
CTTTATT395350.014.6849871
Produced by FastQC (version 0.11.5)