FastQCFastQC Report
Thu 12 Mar 2020
HKVNYDRXX_l02_n02_Emily_2_Mar_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVNYDRXX_l02_n02_Emily_2_Mar_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28854586
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG1534040.5316451256656394No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA1480610.5131281384525843No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC1437080.4980421483087645No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC1162590.40291342249720724No Hit
CTTTATTAGGCAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAG1035460.3588545682131776No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC982060.34034797796093835No Hit
GGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACA975390.3380363870062111No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA879390.3047661123954438No Hit
CTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCA840130.29115995634108216No Hit
CTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGG833560.2888830219224077No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA768250.26624883822627016No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC764430.26492495854905007No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGCCACCACCTTC721200.24994293801338893No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT708920.24568711538609495No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT689520.23896374739183573No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG670910.23251416603239428No Hit
CGGAGAGGCTCTGGAGAGAACCTTCGCGTCCTTCCCCACCACCAAGACCT659780.22865689356970845No Hit
GAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGT652320.22607151598016342No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC648660.2248030867606279No Hit
GCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGA605640.20989384495067784No Hit
GGGAGGTTGAGGTGCGCGAAGCGCAAGAGGGCCCCGGGCCGGGAGCCCGA602330.20874671360732747No Hit
GGGTCTTCCAGTGGTCACCAGGAAATAGGCCAGGGTCTCAGTGGTACTTG582000.2017010398277764No Hit
GTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACACCAGCACGTTGCC577030.19997860998594819No Hit
CTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGA568670.19708132357192717No Hit
CCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGG568480.1970154761534267No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGACGGGGTC556460.19284976051987024No Hit
CTTGAAGTTGACGGGGTCCACACGCAGCTTGTAGGCGTGCAGGTCGCTCA518980.17986049080724983No Hit
GGGGTCCACACGCAGCTTGTAGGCGTGCAGGTCGCTCAGAGCGGACAGCG514580.178335603220923No Hit
CAAGAAGGTGCTGAACTCCTTTAGCGAGGGCCTGAAGAATCTGGACAACC507140.17575715693858857No Hit
CTGGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGA493080.17088444796955327No Hit
GTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAG492830.17079780662942107No Hit
TTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAG481770.16696479374197223No Hit
GAGAACTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCA479580.16620581560241413No Hit
CTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAA478550.16584885328106944No Hit
CCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTCAAGGCCCACGGCAAG475340.1647363784737719No Hit
CTCATAACAGCATCAGGAGAGGACAGGTCCCCAAAGGAGTCAAAGAACCT464130.16085138078224376No Hit
GTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAG456980.15837343845446267No Hit
CGGAGAGGCTCTGGAGAGGACCTTCGCGTCCTTCCCCACCACCAAGACCT442540.15336903464842644No Hit
GCCGAAGTGGTGAGCCAGCACGCACACCAGCACGTTGCCCAGGAGCTTGA442230.15326159938666248No Hit
GGGCAGGCTGCTGGTCGTCTACCCCTGGACTCAGAGGTTCTTTGACTCCT438030.15180602487244144No Hit
CCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACC433550.15025341205727227No Hit
CTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGGATCCTG431350.14949096826410885No Hit
CAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGGCTGCCGTCAC430550.1492137159756858No Hit
CAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAGTTCAGAAGAC430380.14915479986439592No Hit
CTCAGAGCGGACAGCGCGCCCGGCAGGTCGTCCATGTGGGCCACGGCGTT429060.14869733358849785No Hit
CAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGG417040.14453161795494138No Hit
TTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCCA416620.14438606050351926No Hit
GTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGACTCAGAGGTTC412920.14310376866956262No Hit
CTGGTGTGCGTGCTGGCTCACCACTTCGGCAAGGAATTCACCCCTCAGGT411850.14273294373379677No Hit
CGGCGCTGAAGAACTTGTCCAGGGAGGCGTGGACGGCGGGGGTGAATTCC410550.1422824087651093No Hit
GTCAAGTCCACCTGGGACAAGATCGGCGGCCACGCGGGCGAGTATGGCGG408210.14147144582147184No Hit
CTCAGGTGCAGGCTGCCTATCAGAAGGTGGTGGCCGGTGTGGCCAATGCC407530.14123578137631224No Hit
GGACGACCTGCCGGGCGCGCTGTCCGCTCTGAGCGACCTGCACGCCTACA404610.14022381052356808No Hit
CTTGCCGAAGTGGTGAGCCAGCACGCACACCAGCACGTTGCCCAGGAGCT396280.13733692107036297No Hit
TCCACACGCAGCTTGTAGGCGTGCAGGTCGCTCAGAGCGGACAGCGCGCC392720.13610314838688034No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCCCACATGGACG391610.13571846083669334No Hit
CTGAACTCCTTTAGCGAGGGCCTGAAGAATCTGGACAACCTCAAGGGCAC389560.13500800184760925No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA380180.13175721876584887No Hit
CGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGA377460.13081456098521047No Hit
CCTTGCCGAAGTGGTGAGCCAGCACGCACACCAGCACGTTGCCCAGGAGC376940.13063434699773546No Hit
AAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACAC376290.13040907951339173No Hit
GTTCTTTATTAGGCAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCC375210.1300347889240206No Hit
GATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCC366700.1270855177059203No Hit
CTTCGCGTCCTTCCCCACCACCAAGACCTACTTCCCCCACTTCGACCTGA365620.12671122711654917No Hit
AGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGC363340.12592105809454346No Hit
CCAGTGGTCACCAGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCA363110.1258413480616218No Hit
GGAGAAGGCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAG361120.12515168299416946No Hit
GTCGCAGTGCAGCTCACTGAGCTTAGCGAAGGTGCCCTTGAGGTTGTCCA355930.12335300877302485No Hit
GCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGG353100.12237222880272829No Hit
CTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGT352830.12227865615538548No Hit
AGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCAGGGCATTGGCCA350800.12157512847351197No Hit
CCACACGCAGCTTGTAGGCGTGCAGGTCGCTCAGAGCGGACAGCGCGCCC349520.12113152481203508No Hit
GGACAGGTCCCCAAAGGAGTCAAAGAACCTCTGAGTCCAGGGGTAGACGA340930.11815452836509245No Hit
GGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGG333140.11545478420657292No Hit
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA330630.11458490515164556No Hit
GCAGCCTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCAC326280.11307734583334518No Hit
ATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCCAGTG322840.11188516099312601No Hit
GCAACCCCAAGGTCAAGGCCCATGGCAAGAAGGTGCTGAACTCCTTTAGC320080.11092864059806645No Hit
CGCCGCTTCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACGGCGC318960.11054048739427416No Hit
GTGAATTCCGCGGGGTGGTGGCAGGCCAGGGTCACCAGCAGGCAGTGGCT316940.10984042536600594No Hit
TGGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGAC316070.10953891350234586No Hit
CTGGAGAGAACCTTCGCGTCCTTCCCCACCACCAAGACCTACTTCCCCCA313210.10854773657123341No Hit
CTGGAGAGGACCTTCGCGTCCTTCCCCACCACCAAGACCTACTTCCCCCA312310.10823582774675748No Hit
GAAGAATCTGGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGC310620.10765013228746377No Hit
TGGCAGGCCAGGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGAC309650.1073139638877508No Hit
TGAAGGTTCTTTATTAGGCAGAAACCCAACCCTTGATGGTGGACTTTATT304350.10547716747694802No Hit
TGGCTCACCACTTCGGCAAGGAATTCACCCCTCAGGTGCAGGCTGCCTAT303850.10530388479668361No Hit
CCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGAGACCCTG301740.10457263188596778No Hit
GACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAA296540.1027704920112179No Hit
CGCAGCTTGTAGGCGTGCAGGTCGCTCAGAGCGGACAGCGCGCCCGGCAG292730.10145007798760307No Hit
TTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTT289970.10049355759254351No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC289600.10036532840914786No Hit
GCGGGGGTGAATTCCGCGGGGTGGTGGCAGGCCAGGGTCACCAGCAGGCA289460.10031680925867383No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT277200.086.891751
AACCGTG306350.078.497422
ACCGTGT394900.060.6569633
CCGTGTT427050.056.0734984
CGTGTTC475500.050.2837835
ACAGACT243700.049.2940832
CACAGAC291850.042.197491
TGTTCAC576150.041.688617
CTCAGAA563150.041.5981451
GTGTTCA592350.040.719486
ATTAGGC409600.040.05465
AGACTCA314750.039.594782
TATTAGG413100.039.574844
TCAGAAA585650.039.439872
ACAACCG123000.039.4322
TTATTAG425150.038.3338053
TTAGGCA429950.038.2262236
CAGACTC349100.036.4415251
CAGAAAG673400.034.278973
GTTCACT719050.033.2322468
Produced by FastQC (version 0.11.5)