FastQCFastQC Report
Thu 12 Mar 2020
HKVNYDRXX_l02_n02_Emily_2_Mar_7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVNYDRXX_l02_n02_Emily_2_Mar_7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23859083
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT76450.013.0995811
AACCGTG85700.011.7586442
CGCGTAT11903.9908628E-910.3671111
CGGATAT52450.09.6851951
GGGGAAT174850.09.6289331
CGGGAAT75300.09.5410471
CGGAAAT75850.09.4718631
CGGGGAT119450.09.4167571
CGGGTAT38200.09.3086891
CGGGCAT112550.08.7689831
GGCGAAT97250.08.730761
GATTGAG175100.08.69473655
CCGGAAT80500.08.65428451
CGGCTAT42450.08.5476781
CTCGAAT58750.08.5230891
GATCTCG300650.08.463874145
CGCGGAT76550.08.4372441
CCGTATC90700.08.312839145
CGAAGAT134150.08.2766891
CTTTATT337850.08.2697541
Produced by FastQC (version 0.11.5)